| 高效液相色谱法测定党参中党参炔苷的含量 |
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| 引用本文： | 陈璐,王天志. 高效液相色谱法测定党参中党参炔苷的含量[J]. 西南军医, 2008, 10(2): 35-36 |
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| 作者姓名： | 陈璐 王天志 |
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| 作者单位： | 1. 成都中医药大学药学院,四川,成都,610015
2. 四川大学华西药学院,四川,成都,610041 |
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| 摘 要： | 目的分析不同产地党参中党参炔苷的含量。方法采用高效液相色谱，Diamonsil C18（250．0mm×4．6mm，5μm）色谱柱，以乙腈-水（20：80）为流动相，检测波长267nm，柱温30℃。结果党参炔苷在2—500Mg·ml^-1（r=0．9996）范围内与峰面积呈良好线性关系；平均回收率98．3％，RSD1．37％（n=5）。测定了9个产地党参中党参炔苷的含量，含量范围为0．044-0．982mg·g^-1。结论此方法准确、简便，适合于党参中党参炔苷的含量测定。
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| 关 键 词： | 党参 党参炔苷 高效液相色谱法 |
| 文章编号： | 1672-7193(2008)02-0035-02 |
| 修稿时间： | 2007-12-07 |
Content Determination of Lobetyolin in Codonopsis by HPLC |
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| Chen Lu,Wang Tianzhi. Content Determination of Lobetyolin in Codonopsis by HPLC[J]. , 2008, 10(2): 35-36 |
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| Authors: | Chen Lu Wang Tianzhi |
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| Affiliation: | Chen Lu, Wang Tianzhi (Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province 610075) |
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| Abstract: | Objective To determine the content of lobetyolin in codonopsis from different habits by HPLC. Methods Diamonsil- C18 column （ 250.0mm × 4.6mm, 5μm ） was used at the temperature of 30 ℃, the mobile phase consisted of acetonitrile-water （20： 80 ） and the UV detector was set at 267nm for lobetyolin. Results The calibration curve was linear in the range of 2 - 500μg · ml^-1 （ r = 0.9996）, the average recovery was 98.3% with RSD of 1.37% （ n = 5）. The contents of lobetyolin in codonopsis from 9 habits were detected. Conclusion The method is accurate and simple in determining the content of lobetyolin in codonopsis. |
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| Keywords: | codonopsis lobetyolin HPLC |
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