超滤法处理板蓝根水提液的实验研究

目的:观察5种超滤膜对板蓝根水提液中各组分的超滤作用及抗污染情况,以期用超滤法替代醇沉法生产板蓝根制剂。方法:以重量分析法、薄层扫描法、高效液相色谱法测定醇沉液及超滤液中固体物、多糖、氨基酸、氯仿提取物的含量。结果:各种超滤液中固体物含量均低于醇沉液;聚丙烯腈膜和聚砜-6000膜超滤液中多糖含量接近醇沉液;各超滤液中精氨酸含量均高于醇沉液,而氯仿提取物含量均低于醇沉液;聚砜膜抗污染性能最强。结论:根据分离有效成分的需要,可选用聚砜-50000超滤膜超滤法替代醇沉法处理板蓝根水提液,实现高效、节能。

Experimental Study on the Isatis Indigotica Fort. Water-extraction by Ultra-filtration

OBJECTIVE:To observe the ultra-filtration and the resistance to pollution of5ultra-filtrated membrane in respect of each of the compositions from the Isatis indigotica Fort.water-extraction,in order to produce the Isatis indigotica Fort.-preparation through ultra-filtrated method instead of ethanol-sedimentary method.METHODS:By measuring the quantities of the solid residue and the contents of polysaccharide,arginine and chloroform extraction in the ethanol-sediˉmentary solution and ultra-filtrated solution by gravimetry,TLC-scan and HPLC respectively.RESULTS:The solid residue in the ultra-filtered solution was lower than that in the ethanol-sedimentary solution;The content of polysaccharide in the ultra-filtered solution with polypropylene nitrile membrane and polysulfone-6000membrane were close to that in the ethanol-sedimentary solution.The arginine in the ultra-filtered solution was higher than that in the ethanol-sedimentary solution,but the chloroform extraction in the ultra-filtered solution was lower than that in ethanol-sedimentary solution;The resistance to pollution of polysulfone membrane was the strongest in these ultra-filtrated membrane.CONCLUSION:According to the requirement for isolation of active components,ultra-filtrated method with polysulfone-50000membrane could be used instead of ethanol-sedimentary method for efficient and economical application.