人血浆中普罗帕酮对映体浓度的立体选择性HPLC测定法

 目的：建立血浆中普罗帕酮对映体浓度的立体选择性高效液相色谱测定法。方法：血浆标本经碱化以正己烷 异戊醇(99:1)提取、吹干后与GITC柱前衍生化，形成非对映体。以乙晴-水-冰醋酸(60∶40∶0.01)为流动相，经C₁₈ODS柱分离后在紫外λ208nm处检测。结果：对映体曲线范围25μg·L^-1～800μg·L^-1；最低检出限<20μg·L^-1；批内、批间平均RSD均<6%；平均回收率：102.2%；临床大多数常用药物对本法无干扰。结论：本法为普罗帕酮临床药动 药效学研究提供了一种简便、可行的检测方法。

Determination of propafenone enantiomeric concentrations in human plasma by stereoselective high performance liquid chromatography

OBJECTIVE: To establish stereoselectic HPLC method for determining propafenone enantiomers in human plasma. METHOD: The plasma was extracted with hexane-iso-pentanol (99∶1) after basification. A precolumn derivation was performed by using GITC. The enantiomers were eluted with acetonitrile water acetic acid (60∶40∶0.01) on C₁₈ ODS column and detected under λ 208 nm. RESULTS: The range of standard curve of each enantiomer was 25 μg·L^-1～800 μg·L^-1. The lowest limit of detection was 20 μg·L^-1 or less. The average RSD of within or between day was less than 6% and the average recovery rate was 102.2%. Most commonly coadministered drug did not interfere the assay. CONCLUSION: The present study provides a simple and reliable quantitative method for pharmacokinetic and pharmacodynamic studies of propafenone enantiomers.