Immunological adaptive response induced by low dose X-rays

目的：研究低剂量电离辐射能否诱导免疫适应性反应。方法：采用单克隆抗体免疫荧光标记技术，流式细胞术检测 Ｔ Ｃ Ｒ／ Ｃ Ｄ３ 受体。采用３ Ｈ Ｔｄ Ｒ 掺入法测定胸腺细胞自发增殖率及丝裂原诱导的脾细胞增殖率。采用 Ｃ Ｔ Ｌ Ｌ 依赖株检测 Ｉ Ｌ ２ 活性。结果：证明免疫适应性反应的最佳诱导剂量为００２５～０１ Ｇｙ，最佳攻击剂量为１０～１５ Ｇｙ，最佳诱导时间为６～１２ ｈ。结论：低剂量辐射能诱导免疫适应性反应。

Immunological adaptive response induced by low dose X-rays

Objective: To find out whether or not low dose radiation could induce resistance to challenge dose induced depression of immune functions.Methods:The following techniques were employed in this experiment:flow cytometry with immunofluorescence for the expression of TCR/CD3 receptor, 3H TdR incorporation technique for the spontaneous proliferation of thymocytes and the mitogen induced proliferation of splencoytes as well as a bioassay with CTLL cells for IL 2 production.Results:The following optimal conditions for the induction of immunological adaptive response were determined:0.025 to 0.1 Gy for the conditioning doses (D 1),1.0 to 1.5 Gy for the challenge doses (D 2),and 6 to 12 h for the intervals between D 1 and D 2.Conclusion:Immunological adaptive response could be induced by low dose radiation.