复方半枝莲抑制人结肠癌COLO205细胞增殖

目的:探讨复方半枝莲对人结肠癌细胞COLO205体内外生长的抑制作用及机制。方法:体外培养COLO205细胞,分为空白组,顺铂(0.15 g·L~(-1))组,复方半枝莲高、中、低剂量(0.60,0.30,0.15 g·L~(-1))组。利用噻唑蓝(MTT)法检测复方半枝莲对COLO205细胞增殖抑制率的影响。采用免疫印迹法(Western blot)分别检测细胞内凋亡相关蛋白B淋巴细胞瘤-2(Bcl-2),Bcl-2相关X蛋白(Bax),细胞凋亡调控因子(PUMA)的表达情况。选用SPF级裸鼠,于背部接种结肠癌COLO205细胞,待瘤体长到肉眼可见时,测量瘤体大小,根据瘤体大小区间随机分为复方半枝莲高、低浓度组,紫杉醇组和空白组,应用结肠癌移植瘤裸鼠模型体内实验检测复方半枝莲对COLO205细胞体内生长的影响。结果:体外细胞实验表明,经复方半枝莲作用后的人结肠癌细胞COLO205细胞出现了变形,并有细胞膜破损,细胞数逐渐减少,脱落而死亡的现象。48 h时复方半枝莲对COLO205细胞的抑制作用最强。其高、中、低剂量组抑制率分别为68.46%,63.11%,54.91%;且经复方半枝莲干预,COLO205细胞内的Bax,PUMA表达上调,Bcl-2表达下调;体内移植瘤实验显示,与空白组肿瘤体积比较,给药组的肿瘤体积明显减小。结论:复方半枝莲在体外内对人结肠癌细胞COLO205的生长均具有抑制作用,其机制可能与诱导细胞凋亡有关。

Antiproliferation Effect of Compound Banzhilian on Human Colon Cancer COLO205 Cell Line

Objective: To investigate the antiproliferation effect of compound Banzhilian on COLO205 human colon cancer cells in vitro and in vivo. Method: COLO205 cells were cultured in vitro and divided into blank group, cisplatin group (0.15 g · L^-1), and compound Banzhilian high dose group, middle dose group and low dose group (0.60, 0.30, 0.15 g · L^-1). The effect of rate of compound Banzhilian on antiproliferation rate of COLO205 cells was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Western blot assay was used to detect the expression levels of apoptosis-related proteins including Bcl-2, Bax, and p53 up-regulated modulator of apoptosis (PUMA). SPF nude mice were used for in vivo experiment, and they were inoculated with human colon cancer cells COLO205 in their back skin. Tumors size would be measured after they could be seen. According to the volume of tumors, nude mice were randomly divided into compound Banzhilian high and low dose groups, paclitaxel group and blank group. The effect of compound Banzhilian in vivo growth of COLO205 cells was detected with using colon cancer transplantation tumor models in nude mice. Result: In the in vitro experiment, after being treated with compound Banzhilian, most COLO205 cells were deformed with damages of cell membrane, the number of cells decreased gradually, and eventually died. Statistical analysis showed that the antiproliferation effect on COLO205 cells was at 48 h treatment with compound Banzhilian. The inhibition rates were 68.46%, 63.11%, and 54.91% respectively in compound Banzhilian high, middle and low dose groups, the expression levels of Bax and PUMA were up-regulated in COLO205 cells treated with compound Banzhilian, while the expression level of Bcl-2 was down-regulated. The in vivo transplantation tumor experiment showed that tumor volume in treatment groups was significantly smaller than that of in blank group. Conclusion: Compound Banzhilian can inhibit COLO205 colon cancer cells proliferation both in vitro and in vivo, and its potential mechanism may be related to the induction of apoptosis.