补阳还五汤血浆指纹图谱的建立

目的:建立补阳还五汤含药血浆指纹图谱,探讨补阳还五汤在大鼠体内的药效物质基础。方法:通过比较补阳还五汤水煎液、补阳还五汤水煎液含药血浆、空白血浆HPLC指纹图谱,推断大鼠口服补阳还五汤水煎液后,血浆中的原型成分及代谢产物。结果:口服补阳还五汤水煎液后,在大鼠血浆中发现了30个入血成分,其中10个为血浆固有成分,1个为溶剂峰,19个为药源性成分,其中4个为药材原型成分,15个为代谢产物,其中2个为黄芪代谢产物,1个为桃仁代谢产物。结论:该研究建立的方法准确、可靠,可用于补阳还五汤水煎液血浆指纹图谱和药效物质基础的研究。

Estblish Fingerprint of Medicated Plasma of Buyanghuanwu Tang

Objective: In order to establish the plasma HPLC fingerprints of Buyanghuanwu Tang(BYHWT) and to further explore its pharmacodynamic material basis. Method: The prototype components and metabolites in rat plasma were identified through comparing the fingerprints of BYHWT, rat plasma-containing active components and blank plasma. Result: After the ig administration of BYHWT, 30 main components into blood were marked in the plasma fingerprint,10 came from the original compound of rat plasma, one peak was the solvent peak. Other 19 were the drug-induced compositions, 4 of which were prototype constituents, other 15 were metabolites,such as 2 metabolites from Astragali Radix, 1 metabolite from Semen Persicae. Conclusion: The method established in this study was accurate, reliable, and can be used to research the BYHWT plasma fingerprint and efficacy material foundation.