| 消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定 |
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| 引用本文： | 龚晓斌,曾庆花,张凤,高守红,陆文铨,陈万生.消癌平注射液HPLC指纹图谱及7种甾体有效成分的含量测定[J].中国实验方剂学杂志,2016,22(15):68-72. |
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| 作者姓名： | 龚晓斌 曾庆花 张凤 高守红 陆文铨 陈万生 |
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| 作者单位： | 第二军医大学附属长征医院, 上海 200003,第二军医大学附属长征医院, 上海 200003;广西壮族自治区食品药品检验所, 南宁 530021,第二军医大学附属长征医院, 上海 200003,第二军医大学附属长征医院, 上海 200003,第二军医大学附属长征医院, 上海 200003,第二军医大学附属长征医院, 上海 200003 |
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| 基金项目： | 国家自然科学基金项目（81202866） |
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| 摘 要： | 目的:建立消癌平注射液HPLC指纹图谱,并准确测定消癌平注射液中tenacissoside H(TSH)等7种甾体有效成分的含量。方法:采用Agilent Eclipse XDB-C18色谱柱,流动相乙腈-水,梯度洗脱,柱温30℃,流速0.8 m L·min-1,平衡时间10min,进样20μL,蒸发光散射检测器漂移管温度60℃,载气压力0.4 MPa,检测17β-tenacigenin B(17β-TB),tenacigenin B(TB),tenacigenin A(TA),tenacigenoside A(TSA),marsdenoside I(MSI)和tenacissoside F(TSF)的含量;并运用《中药色谱指纹图谱相似度评价系统》软件建立消癌平注射液的对照指纹图谱,计算其相似度。结果:11批消癌平注射液的HPLC指纹图谱有13个共有峰,相似度均在0.94以上。TSH等7种甾体在标准曲线范围内均呈现良好的线性(r0.999),精密度、重复性和准确度均符合要求。结论:所建立的HPLC-ELSD方法操作简便、结果可靠,具有良好的精密度、稳定性和重复性,结合指纹图谱研究为消癌平注射液中多成分含量测定和质量控制提供了依据。
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| 关 键 词： | 消癌平注射液 高效液相色谱-蒸发光散射 指纹图谱 含量测定 |
| 收稿时间： | 7/9/2015 12:00:00 AM |
Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD |
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| GONG Xiao-bin,ZENG Qing-hu,ZHANG Feng,GAO Shou-hong,LU Wen-quan and CHEN Wan-sheng.Fingerprint Analysis of XiaoaipingInjection and Simultaneous Determination of Its Seven Active Steroidal Components in XiaoaipingInjection by HPLC-ELSD[J].China Journal of Experimental Traditional Medical Formulae,2016,22(15):68-72. |
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| Authors: | GONG Xiao-bin ZENG Qing-hu ZHANG Feng GAO Shou-hong LU Wen-quan and CHEN Wan-sheng |
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| Institution: | Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China,Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Guangxi Institute For Food And Drug Control, Nanning 530021, China,Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China,Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China,Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China and Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China |
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| Abstract: | |
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| Keywords: | Xiaoaiping injection HPLC-ELSD fingerprint quantitative determination |
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