三硝基甲苯对恒河猴外周血淋巴细胞姐妹染色单体互换的影响

目的了解三硝基甲苯(TNT)对恒河猴外周血淋巴细胞姐妹染色单体互换(SCE)的影响,探讨TNT致突变性。方法给3组恒河猴不同剂量TNT经口染毒,取外周血按微量全血培养法加5-BrdU体外培养,制片后分析SCE频率情况。结果与对照组比较,60、120mg/kg组染毒后SCE频率均显著增加,差异有显著性(P<0·01);60mg/kg组从染毒前的(8·31±5·22)次/细胞升高到染毒后60d的(19·35±11·61)次/细胞和90d的(23·23±9·92)次/细胞,并呈剂量-反应关系;120mg/kg组染毒后SCE频率已达本次实验的最大值(28·85±9·39)次/细胞,和90d(27·58±8·57)次/细胞差异无显著性(P>0·05)。结论TNT可能存在致突变性。

Study on the impact of trinitrotoluene on sister chromatid exchange of Mocaca mullatta peripheral blood lymphocyte.

Objective Study on the affecting of TNT on sister chromatid exchange of Mocaca Mulatta peripheral blood lymphocyte, research the mutation of TNT. Methods Gave Mocaca Mulatta different doses TNT through oral intubations. And SCE changes of peripheral blood lymphocyte were analyzed after 56h incubation in vitro. Results Compared with control group, the SCE frequencies of 60mg/kg group and 120mg/kg have significantly increased, and have a dose-response relation. And the SCE frequencies of the same group increased in company with times. Conclusion The results prompt that TNT have mutation on Mocaca Mulatta peripheral blood lymphocyte.