HBV-DNA 和 ALT、TBA 水平与 HBV 感染模式的相关性研究

目的：探讨乙型肝炎病毒（HBV）患者血清 HBV-DNA 和丙氨酸转氨酶（ALT）、总胆汁酸（TBA）与 HBV 感染模式的关系以评价 HBV 感染性。方法对308例患者采用荧光定量 PCR 法检测血清 HBV-DNA，用 ELISA 方法检测血清乙肝标记物，用连续监测法检测血清 ALT，用循环酶法检测血清 TBA。结果大三阳（HBsAg、HBeAg、HBcAb 阳性者）、小三阳（HBsAg、HBeAb、HBcAb 阳性者）和1，5（HBsAg、HBcAb）阳性3种感染模式间 HBV-DNA 水平和血清 ALT 活性差异有统计学意义（P ＜0．05），部分 HBeAg 阴性患者体内仍存在活跃的病毒复制；在 HBeAg 阴性患者中 HBV-DNA 拷贝数与血清 ALT 水平存在显著正相关关系（P ＜0．05）；3种感染模式间血清 TBA 水平差异无统计学意义（P ＞0．05）。结论血清 HBV-DNA 水平和 ALT 活性与不同 HBV 感染模式存在相关性，它们可在一定程度上反映 HBV 复制及传染性强弱。

Correlation of HBV-DNA load,ALT and TBA levels with hepatitis B virus infection pattern

Objective To explore the correlation of serum HBV-DNA load,transaminase（ALT）and total bile acid （TBA）levels with hepatitis B virus （HBV）infection patterns to assess HBV infectivity.Methods The fluorescent quantitative PCR was used to detect serum HBV-DNA in 308 patients with HBV infection.ELISA was adopted to detect the HBV markers.The continuous mo-nitoring method was adopted to detect serum ALT and the cycling enzymatic method was adopted to detect serum TBA.Results The HBV-DNA content and serum ALT level had staitstical differences among the 3 infection patterns of HBsAb,HBeAg and HB-cAb positive,HBsAg,HBsAb,and HBcAb positive and 1,5 positive （P 〈0.05）,the partial patients with HBeAg negative had the active HBV replication in vivo;HBV-DNA copy number in the patients with HBeAg negative was positively correlated with ALT level （P 〈0.05）.Serum TBA level had no statistical difference among three knds of HBV infection patterns （P 〉0.05）.Conclusion Serum HBV-DNA content and ALT activity have the correlation with the different HBV infection patterns,which could reflect the HBV replication and the infectivity intensity in some extent.