环孢菌素-A对大鼠骨髓来源树突状细胞免疫刺激活性的影响

目的 研究环孢菌素-A（CsA）对体外培养的大鼠树突状细胞前体（DCp）的表型及功能的影响。方法 在大鼠骨髓来源的DC前体培养过程中加入CsA（1mg／L），并设对照组，培养期间观察DC形态，流式细胞仪分析细胞表型，混合淋巴细胞反应检测其刺激同种T细胞增殖能力。结果 CsA显著抑制了DCD的成熟过程，抑制其表面共刺激分子的表达，并显著抑制其刺激同种T细胞增殖能力。结论 CsA可抑制DC成熟，使DC处于未成熟状态，从而发挥免疫抑制作用。

Influence of CsA upon the maturation of cultured dendritic cell progenitors

Objective To explore the influence of Cs A on the maturation of cultured dendritic cell progenitors.Methods Bone marrow-derived DC progenitors were assigned to two groups:The cells in one group were cultured with grantdocyte-macrophage colony-stimulating factor(10 mg/L)and inter- lenkin-4(10 mg/L)for 13 days(control group),and those in other group with the same cytokines and CsA(1 mg/L)for 13 days(CsA group).Cell morphology was monitored under light and electron mi- croscopy and cell surface molecules including CDS0,CD86 and OX62 were assayed by flow cytometry.T cell proliferation was assayed by MTT.Results Compared with DCs in control group,DCs in CsA group were characterized as follows:(1)Lower expression levels of CDS0 and CD86(14.1%,17.1%);(2) Weaker activity of stimulating proliferation of allogenetic T cells.Conclusion The immature DCs inhib- ited by CsA may have the properties of inducing allogenetic-specific immune inhibition in vitro.