| SDF-1/CXCR4轴活化诱导内皮祖细胞增殖、迁移及管型形成 |
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| 引用本文： | 陈飞兰,张华蓉,徐承平,卞修武.SDF-1/CXCR4轴活化诱导内皮祖细胞增殖、迁移及管型形成[J].基础医学与临床,2008,28(5):428-431. |
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| 作者姓名： | 陈飞兰 张华蓉 徐承平 卞修武 |
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| 作者单位： | 第三军医大学,西南医院,病理学研究所,重庆,400038 |
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| 摘 要： | 目的观察趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成的作用。方法用免疫细胞化学检测内皮祖细胞SDF-1和CXCR4表达;用MTT法、Millicell趋化法及Matrigel体外三维成型法分别检测不同浓度的趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成。并应用CXCR4受体抑制剂AMD3100观察上述指标的变化。结果免疫细胞化学显示内皮祖细胞表达SDF-1和CXCR4蛋白。SDF-1可促进内皮祖细胞的增殖、迁移和体外小管样结构的形成。AMD3100可抑制SDF-1的诱导作用。结论SDF-1/CXCR4轴在内皮祖细胞参与血管新生中可能发挥重要作用。
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| 关 键 词： | 内皮祖细胞 趋化因子SDF-1 血管新生化 |
| 收稿时间： | 2007-11-2 |
| 修稿时间： | 2007-12-25 |
Activation of SDF-1/CXCR4 axis induces proliferation, migration,and tubulogenesis of human endothelial progenitor cells |
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| CHEN Fei-lan,ZHANG Hua-rong,XU Cheng-ping,BIAN Xiu-wu.Activation of SDF-1/CXCR4 axis induces proliferation, migration,and tubulogenesis of human endothelial progenitor cells[J].Basic Medical Sciences and Clinics,2008,28(5):428-431. |
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| Authors: | CHEN Fei-lan ZHANG Hua-rong XU Cheng-ping BIAN Xiu-wu |
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| Abstract: | Objective To explore the effect of chemokine SDF-1 on the proliferation,migration and in vitro of human endothelial progenitor cells(EPCs).Methods The expression of CXCR4 and SDF-1 on EPCs was detected with immunocytochemistry.Proliferation,migration and in vitro tubulogenesis of EPCs was detected by MTT,Millicell chemotaxis and three-diamensional in vitro Matrigel assays,respectively.Results SDF-1 and CXCR4 were expressed on EPCs.SDF-1 induced proliferation,migration and tubulogenesis of EPCs.However,AMD3100 abolished the effects induced by SDF-1.Conclusion The SDF-1/CXCR4 axis possibly plays a critical role in regulating vasculogenesis of EPCs. |
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| Keywords: | EPCs SDF-1 neovascularization |
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