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Histochemical characteristics of diphosphate nucleoside consumption in cat and rat nervous system. |
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| Authors: | R Martinez-Murillo R Martinez-Rodriguez A Toledano M A Barca |
| Affiliation: | Instituto Cajal, Madrid, Spain |
| Abstract: | Consumption of diphosphate nucleosides is investigated by histochemical methods in rat and cat nervous system. Results show NDP-ase is effectively in histological sections from animals previously perfused with glutaraldehyde or formaldehyde. Histochemical reaction is increased in presence of Ca++, Mg++, Mn++ or imidazole and inhibited by L-DOPA and Noradrenaline in incubation medium. A comparative study of TPP-ase and NDP-ase activities to dilucidate the identity of both enzymes is described. |
| Keywords: | TPP Thiaminepyrophosphate NDP Nucleoside diphosphate TPP-ase Thiaminepyrophosphatase NDP-ase Nucleoside diphosphatase |
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