湖北地区医务人群严重急性呼吸道综合征冠状病毒血清学调查分析

目的 确定医务人群中是否存在严重急性呼吸道综合征(SARS)冠状病毒(SARS-CoV)隐性或亚临床感染及健康带毒者。方法 以问卷调查方式抽样调查湖北地区有可能接触SARS—CoV的高危医务人群的流行病学状况；以酶联免疫吸附法测定血清SARS-CoV IgM、IgG；以一步法双重RT—PCR检测受试者喉漱液是否存在SARS-CoV。结果 接受调查的102名医务人员中，均曾在抗SARS一线工作过，9名曾到过SARS流行疫区，84名(82．3％)在2003年SARS流行期间，否认曾与确诊SARS患者有过接触；18名报告与确诊SARS患者有过接触，其中1名曾无防护接触过数天，但无任何不适，其他17名均为有防护接触，其中3名在接触后20天内曾有过感冒样不适。全部102名受调查者的血清SARS—CoV IgM、IgG以及喉漱液SARS-CoV检测均为阴性。结论 本次抽样调查未发现湖北地区医务人群中曾经有过SARS-CoV隐性或亚临床感染，也未发现有上呼吸道SARS—CoV健康带毒的情况。

Survey for occult or subclinical infection and healthy carrier of SARS coronavirus among medical staff with exposure potential to SARS coronavirus

Objective To clarify if there had been occult or subclinical infection of SARS and if there are healthy carriers of SARS coronavirus (SARS-CoV) among medical staffs with exposure potential to SARS-CoV in Hubei province.Methods A self-report standard questionnaire was used to evaluate the epidemiological condition of the subjects mentioned above.Serum antibodies of SARS-CoV were tested by ELISA method.Nasopharyngeal aspirates were detected by one-step duplex RT-PCR method for SARS-CoV.Results One hundred and two medical staffs have been investigated.Forty-two reported they experienced 1 or 2 episodes of flu-like illness per year,one reported she had closely contacted a SARS patient without medical protection,and seventeen reported they had closely contacted SARS patients under proper medical protection and among the latter,3 reported they had flu-like illness several days afterward.Of all subjects investigated,neither their serum SARS-CoV IgM or IgG nor their nasopharyngeal aspirates tested for SARS-CoV is positive.Conclusion The chance for occult or subclinical infection of SARS and healthy upper airway carrier of SARS-CoV among medical staffs with exposure potential to SARS-CoV,at least in Hubei province,is very low. [