Characterization of petunia flower mottle virus (PetFMV), a new potyvirus infecting Petunia x hybrida |
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Authors: | A Feldhoff T Wetzel D Peters R Kellner G Krczal |
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Institution: | InnovaPlant GmbH, Gensingen, Federal Republic of Germany, DE Zentrum Grüne Gentechnik, Staatliche Lehr-und Forschungsanstalt, Neustadt/Weinstrasse, Federal Republic of Germany, DE Department of Virology, Agricultural University of Wageningen, Wageningen, The Netherlands, NL Institut für Physiologische Chemie und Pathobiochemie, Johannes Gutenberg-Universit?t Mainz, Mainz, Federal Republic of Germany, DE
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Abstract: | Summary. With the introduction of cutting-grown Petunia x hybrida plants on the European market, a new potyvirus which showed no serological reaction with antisera against any other potyviruses
infecting petunias was discovered. Infected leaves contained flexuous rod-shaped virus particles of 750 – 800 nm in length
and inclusion bodies (pinwheel structures) typical for potyviruses in ultrathin leaf sections. The purified coat protein with
a Mr of approximately 36 kDa could be detected in Western immunoblots with a specific antibody to the coat protein of the petunia-infecting
virus. The 3′ end of the viral genome encompassing the 3′ non-coding region, the coat protein gene, and part of the NIb gene
was amplified from infected leaf material by IC/PCR using degenerate and specific primers. Sequences of PCR-generated cDNA
clones were compared to other known sequences of potyviruses. Maximum homology of 56% was found in the 3′ non-coding region
between the petunia isolate and other potyviruses. A maximum homology of 69% was found between the amino acid sequence of
the coat protein of the petunia isolate and corresponding sequences of other potyviruses. These data indicate that the petunia-infecting
virus is a previously undescribed potyvirus and the name petunia flower mottle virus (PetFMV) is suggested.
Accepted November 5, 1997 Received July 25, 1997 |
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