查体儿童外周血白细胞形态参数的变化及临床意义研究

目的 分析儿童外周血白细胞形态参数的变化及其临床意义。方法 设定运用Sysmex XE-2100全自动血细胞分析仪(简称XE-2100分析仪)检验血常规的180例门诊表观健康查体儿童(年龄≤15岁)为研究对象,回顾性收集其白细胞形态参数数据。按照年龄0～岁、1～岁、2～岁、6～岁、12～15岁分组,分析研究各白细胞形态参数的变化。结果 研究观察各年龄组儿童中性粒细胞散射荧光度即颗粒度(NEUT-X)为1 355.0±41.5、1 348.3±22.5、1 348.4±51.3、1 339.3±51.6、1 335.8±46.2,各组之间差异无统计学意义(P=0.292);中性粒细胞核酸荧光强度即核的大小(NEUT-Y)为495.6±28.2、477.6±38.6、486.5±40.2、481.3±32.2、479.9±39.6,各组之间差异无统计学意义(P=0.412);淋巴细胞核酸荧光强度(LYMPH-Y)为713.6±46.3、708.3±60.3、711.0±61.2、712.8±72.3、711.6±75.3,各组间差异无统计学意义(P=0.115);淋巴细胞散射荧光强度(LYMPH-X)为896.3±36.2、854.6±19.3、858.4±17.6、860.1±15.7、851.3±18.2,各组间差异有统计学意义(P=0.008),其中0～岁组与其他各组两两比较差异有高度统计学意义(P＜0.001)。结论 小于1岁的健康儿童外周血淋巴细胞颗粒度较其他年龄健康儿童高,具有较高的自身免疫抵抗能力。

Study on the changes of parameter of peripheral blood leucocytes in physical examination of children

Objective To analyze the morphological parameters of peripheral blood leucocytes in children,and to find the clinical significance.Methods A total of 180 healthy children (under 15 years old) were enrolled in this study,and parameters of white blood cells were analyzed by Sysmex XE-2100 automatic blood analyzer.The parameter data and changes of white blood cells were analyzed by age 0～,1～,2～,6～,12～15 years old.Results The fluorescence of neutrophilic granulocytes (NEUT-X) of all age groups were 1 355.0±41.5,1 348.3±22.5,1 348.4±51.3,1 339.3±51.6,1 335.8±46.2 respectively,and there were no statistical difference among groups (P=0.292).The fluorescence intensity of neutrophilic nucleic acid (NEUT-Y) were 495.6±28.2,477.6±38.6,486.5±40.2,481.3±32.2,479.9±39.6 respectively,and there were no statistical differences (P=0.412).The fluorescence intensity of lymphocyte nucleic acid (LYMPH-Y) were 713.6±46.3,708.3±60.3,711.0±61.2,712.8±72.3,711.6±75.3,and there were no significiant differences among groups (P=0.115).The fluorescence intensity of lymphocyte(LYMPH-X) were 896.3±36.2,854.6±19.3,858.4±17.6,860.1±15.7,851.3±18.2,and there were statistical differences among groups (P=0.008).Conclusion The granularity of peripheral blood lymphocyte in healthy children under 1 year old is higher than that of other ages,which indicated 0~1 years old children have better immunity.