Abstract: | A series of new cyclic phosphoramidate mustard-quinazolineconjugates were designed and synthesized based on the drug candidate EMB-3, a multi-target-directed ligand against tumor cells, and their anti-tumor activities were evaluated on breast cancer and lung cancer cells. Compound 6d exhibited the best anti-tumor performance with IC50 = 0.6 μM(8-fold of EMB-3) on BT474breast tumor cells. Compound 6d inhibited epidermal growth factor receptor (EGFR, biomarker for NSCLC) and human epidermal growth factor 2 (HER2, biomarker for breast cancer) with IC50 of 18 nM and 78 nM, respectively. The preliminary pharmacokinetic study revealed that 6d was more stable than EMB-3 during the in vivo metabolism. A single dose per os (PO) administration of 6d in rat model (10 mg/kg) resulted in a moderate t1/2of 1.7 h. These results indicated that compound 6d was a potential lead compound for the treatment of breast cancer. |