双歧杆菌在新生鼠坏死性小肠结肠炎应用中剂量的研究

目的 研究双歧杆菌在预防新生鼠坏死性小肠结肠炎中应用剂量的问题,为临床合理用药提供理论依据。方法 选取新生SD大鼠40只,随机分为5组:A,正常对照组;B,NEC模型组;C,NEC模型组,每日给予0.4 mg/g的酪酸梭菌二联活菌散(主要有效成分为双歧杆菌)灌胃;D,NEC模型组,每日给予4 mg/g酪酸梭菌二联活菌散灌胃;E,NEC模型组,每日给予20 mg/g酪酸梭菌二联活菌散灌胃。每日记录体重;3 d后禁食1 d,处死所有新生鼠,取血检测TNF-α并对比;HE染色法观察肠道组织。结果 体重变化均值为A>D>E>C>B,除B与C间,D与E间差异无统计学意义,其余组两两对比差异均有统计学意义。HE染色评分均值为B>D>C>E>A,仅A与其他各组间差异有统计学意义。TNF-α均值为B>C>D>E>A,除B与C间,D与E间差异无统计学意义,其余组间均有统计学意义。综合分析,D、E的炎症程度低于B、C。结论 酪酸梭菌二联活菌散在新生鼠NEC中应用的最佳预防剂量可能为4~20 mg/g,相当于双歧杆菌4.0×10³~20×10³CFU。

Research of the dose of bifidobacterium in the application in neonatal necrotizing enterocolitis in newborn rats

Objective To study the dose of bifidobacteria in preventing neonatal necrotizing enterocolitis (NEC) in newborn rats,and to provide theoretical for clinical rational drug use. Methods A total of 40 SD rats were divided into 5 groups randomly:A,the normal group;B,NEC model group,give them lavage with normal saline daily while making model;C,NEC model group,establish the model and give 0.4 mg/g of duplex butyric acid bacteria living bacterium(Its main effective components is bifidobacterium)to fill the stomach daily;D,NEC model group,established the model and give 4 mg/g of duplex butyric acid bacteria living bacterium to fill the stomach daily;E,NEC model group,established the model and give 20 mg/g duplex butyric acid bacteria living bacterium to fill the stomach daily;Weighed their body weights and recorded everyday.Three days later,to fast one day.Put all the newborn mice to death.Anatomy all the rats and detect the contents of TNF-α and contrast them,observe the changes of their intestinal tissue and compared them;Observe the pathological changes of the intestinal tissue with HE staining. Results The average changes of body weight were A>D>E>C>B.There was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups.The mean scores of their HE staining pathology were B>D>C>E>A,there was statistical significance between A and other groups only.The averages of their TNF-α were group B>group C>group D>group E>group A.Based on the analysis of the above data,there was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups. Conclusion The optimal dose of duplex butyric acid bacteria living bacterium in neonatal necrotizing enterocolitis mice model may be 4 to 20 mg/g.The equivalent dose of bifidobacterium is 4×10³ CFU to 20×10³CFU.