金黄地鼠实验性肺气肿肺泡Ⅱ型上皮表面活性物质蛋白A和B的 …

目的 观察金黄地鼠实验性肺气肿肺泡Ⅱ型上皮表面活性物质蛋白（ＳＰ）Ａ和Ｂ的变化。方法 金黄地鼠气管注入弹性蛋白酶后,分别于注入弹性蛋白酶的３０、６０和９０ｄ处死,肺组织经光镜检查和图像分析后,再行ＳＰ－Ａ和ＳＰ－Ｂ免疫组化染色和形态定量;同时进行透射电镜观察。结果 注入弹性蛋白酶后第３０、６０和９０ｄ肺平均内衬间隔明显增大（Ｐ〈０．０１）;免疫组化显示注入弹性蛋白酶后３０ｄ肺泡Ⅱ型上皮细胞ＳＰ－Ａ

Changes of surfactant A and B in alveolar type II cells in hamster with elastase-induced emphysema

Objective　To observe the changes ofsurfactant A and B (SP-A and SP-B) in alveolar type II cells of lungs in hamsters withelastase-induced emphysema. Methods　After intratracheal instilling of elastase, hamsters werekilled at the 30th, 60th and 90th day of the experiment respectively. Slides of lungtissue were examined under light microscope and measured by calculating the mean linearintercepts. Then the slides were stained under immunohistochemistry procedures andmeasured by using a morphometric analysis system. Meanwhile, slides were also checked byelectron microscopy. Results　In comparison with the normal group, MLI of the elastasegroup was significantly increased (P<0.01). Immunohistochemistry showed that thepercentage of both SP-A and SP-B positive cells was significantly decreased 30 days afterthe intratracheal instilling of elastase (from 48.0%±3.0% to 9.5%±4.0% and from 28.0%±4.0%to 13.3%±4.1%, respectively; P<0.01), however, a time-dependent recovery 90 days afterinstilling was seen. Electron microscopy showed a significantly decrease of the number oflamellae. Conclusion　The decrease of SP-A and SP-B in alveolar type II cellsseems to play an important role in the development of emphysema.