肝细胞癌组织中中期因子表达及其与乙型肝炎病毒感染的关系

目的　探讨肝细胞癌 (HCC)组织中中期因子 (midkine ,MK)的表达及其与乙肝病毒(HBV)感染的关系。方法　采用原位杂交及免疫组织化学方法检测 6 2例HCC、癌旁肝组织及 10例正常肝组织中MKmRNA、MK蛋白表达及HBVDNA水平。结果　 6 2例HCC组织中MKmRNA、MK蛋白表达阳性率分别为 74 2 %、75 8% ,明显高于无表达的癌旁肝组织及正常肝组织 (均P <0 0 1)。肝癌组织HBVDNA核型阳性率 (6 2 9% ,39/ 6 2 )明显高于癌旁肝组织 (6 5 % ,4 / 6 2 ) (P <0 0 1)。肝癌组织MKmRNA、MK蛋白表达与HBVDNA核型存在密切相关。结论　MKmRNA及其蛋白在HCC组织中呈过度表达 ,并可能与HBVDNA核型存在有密切联系

Expression of midkine and its relationship with HBV infection in hepatocellular carcinomas

Objective To investigate expression of midkine (MK) in hepatocellular carcinoma(HCC) and its relationship with HBV infection. Methods Expression of MK mRNA?MK protein and HBV DNA were determined in 62 cases of human HCC and 10 cases of controls with in situ hybridization and immunohistochemistry. Results Positive rates of MK mRNA and MK protein in carcinoma tissue were 74.2% and 75.8% respectively,which were higher than in paratumorous liver tissue and normal controls ( P <0.01). Positive rate of HBV DNA in the nuclei of carcinoma tissue (62.9%) were higher than in paratumorous liver tissue (6.5%)( P <0.01). There was a significant relationship between expression of MK mRNA and MK protein in the carcinoma tissue and HBV DNA in the nuclei of carcinoma tissue. Conclusions HCC overexpresses MK at the mRNA and protein level. The overexpression of the MK protein might be correlated with the existencr of HBV DNA in the nuclei of carcinoma tissue.