miRNA Let-7对神经母细胞瘤细胞的抑制作用及其机制

为研究miRNA Let-7神经母细胞瘤(NB)的抑制作用,本研究采用小RNA干扰技术(siRNA)降低miRNA Let-7的表达,PCR法检测miRNA Let-7表达,Western blotting检测相关凋亡蛋白的表达, 采用吸光度值反映细胞的增殖情况,Transwell方法检测细胞转移。结果显示,与未转染组和si-NC(携带空质粒的干扰RNA)转染组细胞相比,siRNA-Let-7转染组细胞的数量增多,Cleaved casp-3、Bax mRNA及其蛋白显著增高,而Bcl-2 mRNA及蛋白表达显著降低。结果表明,miRNA Let-7能够通过抑制细胞的增殖、迁移和侵袭能力和促进细胞的凋亡,进而对神经母细胞瘤有重要抑制作用。

The inhibitory effects of miRNA Let-7 on neuroblastoma cells

To explore the miRNA Let-7 on the proliferation, metastasis and apoptosis of neuroblastoma(NB), The technology of siRNA was used to interfere on the expression of miRNA Let-7 in this study, the PCR was used to test the expression of miRNA Let-7, Western blotting was used to test the expression of related proteins of apoptosis. the absorbance of cells was used to analyze the proliferation of cells, Transwell was test immigration. The results showed, compared to the no transfection group and si-NC group, the number of cells was increased, the expression of Cleaved casp-3, Bax mRNA and protein levels were significantly increased in siRNA-Let-7 group, while bcl-2 mRNA and protein levels were lower. Overall, miRNA Let-7 has an important inhibitory effect on neuroblastoma by inhibiting cell proliferation, migration and invasion and promoting cell apoptosis.