MiR-4262经Kaiso-β-catenin途径抑制宫颈癌Hela细胞的增殖

分析miR-4262抑制宫颈癌细胞增殖的Kaiso-β-catenin机制。将miR-4262表达载体pGL3-miR-4262表达载体瞬时转染Hela宫颈癌细胞株作为miR-4262组,以未转染Hela细胞和转染pGL3空载体细胞分别作为空白组和阴性组,分析各组细胞的增殖情况,免疫印迹法分析各组细胞中细胞凋亡蛋白、Kaiso、β-catenin及Axin蛋白的表达。结果显示:与空白组和阴性组相比,miR-4262组细胞的抑制率明显升高(P<0.05),Bax和Caspase3表达明显升高而Bcl2表达明显降低(P<0.05),且Kaiso、β-catenin表达明显升高而Axin表达明显降低(P<0.05)。因此,miR-4262能明显抑制宫颈癌细胞的增殖,且其作用可能与调节Kaiso-β-catenin信号通路蛋白表达有关。

Mi-4262 inhibiting proliferation of cervical cancer cells through kaiso-beta-catenin

The aim of this study was to analyze the Kaiso-beta-catenin mechanism of microRNA-4262 inhibiting the proliferation of cervical cancer cells. The expression vector pGL3-miR-4262 was transfected into Hela cervical cancer cell line as the group of microRNA-4262. Non-transfected Hela cells and pGL3 empty vector cells were used as blank group and negative group, respectively. The proliferation of cells in each group was analyzed. The expression of apoptotic protein, Kaiso, beta-catenin and Axin protein were analyzed by Western blot. The results showed that compared with the blank group and the negative group, the inhibitionrate of the cells in the microRNA-4262 group increased significantly (P<0.05), the expression of Bax and Caspase-3 increased significantly, and the expression of Bcl-2 decreased significantly (P<0.05). The expression of Kaiso and beta-catenin increased significantly, while the expression of Axin decreased significantly (P<0.05). So Mi-4262 can significantly inhibit the proliferation of cervical cancer cells, and its effect may be related to the regulation of Kaiso-beta-catenin signaling pathway protein expression.