拓扑替康对小鼠Lewis肺癌的抑制作用

目的：探讨拓扑替康对小鼠Lewis肺癌的抑制作用及抗血管生成作用。 方法：将Lewis肺癌模型小鼠随机分为对照组10只及拓扑替康小、中、大（0.312、0.624 和1.248 mg?kg^-1）剂量组各15只，第21天处死小鼠，观察肿瘤生长情况，绘制肿瘤生长曲线，计算原发瘤抑瘤率，免疫组织化学染色计数肿瘤组织微血管密度（MVD）及检测血管内皮细胞生长因子（VEGF）的表达。 结果：拓扑替康各剂量组较对照组小鼠的肿瘤生长缓慢，原发瘤抑瘤率与对照组相比差异有显著性（P<0.05）；拓扑替康大、中剂量组MVD与对照组比较差异有显著性（P<0.05）；各剂量组VEGF阳性率均较对照组低。 结论：拓扑替康明显地抑制小鼠Lewis肺癌原发瘤的生长，并可抑制肿瘤的微血管生成。

Inhibitory effects of topotecan on mice with Lewis lung cancer

Objective To explore the inhibitory effects of topotecan on mice with Lewis lung cancer(LLC) and its mechanisms. Methods LLC cells were inoculated subcutaneously into C57BL/6 mice. Fifty-five mice were randomly divided into control group (n=10) and experimental groups (Topotecan of 0.312,0.624, and 1.248 mg·kg -1 , 15 in each group).Topotecan and phosphate- buffered saline vehicle were injected intraperitoneally, respectively.On the 21th day ,the mice were killed. Tumor growth curve was described, the inhibitory rate of primary tumor was measured. Microvessel density (MVD) was quantitated by immunohistochemistry using monoclonal antibodies against factor F8, vascular endothelial growth factor (VEGF) immunoreactivity was examined by immunohistochemistry using polyclonal antibodies against VEFG. Results Tumor growth curves in experimental groups were smooth compared with control group.There were significant differences of inhibitory rates of primary tumor between control and experimental groups(P<0.05);there were significant differences of MVD between high,middle dosages of topotecan groups and control (P<0.05).VEGF-positive rates were not significantly different between control and experimental groups. Conclusion Topotecan can effectively suppress the growth of the implanted LLC in C57BL/6 mouse and can inhibit angiogenesis.