胃癌组织中HPV16感染与 P21基因失活的相关研究

目的:研究胃癌组织中HPV16的感染及P21基因突变是否存在协同致癌作用及其与胃癌患者预后的关系。方法:采用PCR技术,检测46例胃癌组织标本中HPV16型感染,并采用免疫组化SP法对胃癌P21基因突变进行检测,经近3年门诊或书信定期随访。结果:HPV 16感染19例(41.30%),P21阳性表达24例(52.17%)。随访发现46例有21例出现复发或远处转移,其中HPV 16阳性者复发率为73.68%(14/19),P21基因阳性表达者复发率为66.6%(16/24)。值得提出的是两项同时阳性者8例中有6例出现复发或远处转移。结论:HPV16与胃癌发生有关,P21基因失活具有协同致癌作用,亦可作为判断胃癌预后的指标之一。

Correlation of HPV16 Infection and P21 Gene Mutation in Gastric Cancer

Objective: To analyze whether there is a synergistic carcinogensis between the infection of humam papilloma virus(HPV) and the p21 gene mutation in gastric cancer tissue and the relation with the prognosis.Methods: The infection of HPV16 was detected by PCR and the p21 gene mutation was detected by immunohistochemical method in 46 specimens of gastric cancer.(Results:) The positive rate of HPV16 was 41.3% and the gene mutation rate of p21 was 52.17%.The recurrence or remote metastasis were observed in 21 of 46 patients.The recurrence rate was 73.68% in patients with HPV16 positive and 66.6% in those with p21 gene mutation.It also should be introduced that the recurrence or remote metastasis were observed in 6 of 8 patients with both HPV16 positive and p21 gene mutation.Conclusion: The infection of HPV16 may be one of the causes of gastric cancer and there is synergistic carcinogensis in the infection of HPV16 and the p21gene mutation.They could be one of the prognosis markers in gastric cancer.