同种异体骨髓单个核细胞肝内移植治疗急性肝损伤的磁共振扩散成像实验观察

目的 探讨骨髓单个核细胞肝内移植治疗急性肝损伤的疗效及利用磁共振扩散成像评价骨髓单个核细胞移植后急性肝损伤模型肝组织修复情况的可行性.方法 10只健康同龄兔作正常对照,仅行正常肝脏平均ADC值的测定.42只肝损伤模型兔随机均分为两组:(1)移植组:肝内注射5 ml骨髓单个核细胞悬液;(2)对照组:肝内注射5 ml D-hanks液.移植术前均行肝功能测定,术后第7、14、28天,两组分别选7只行肝脏磁共振扩散成像及肝功能检查后处死,行肝脏病理切片.结果 术前,移植组、对照组肝功能差异无统计学意义(P＞0.05),术后各时点移植组肝功能好于对照组(P＜0.05).术后第7天,移植组、对照组平均ADC值低于正常对照组(P＜0.05),并随时间推移逐渐向正常肝脏平均ADC值接近;同时点,移植组平均ADC值高于对照组(P＜0.05);移植组内各时点平均ADC值两两比较差异有统计学意义(P＜0.05).对照组内部比较,术后第7天平均ADC值小于术后第14天及28天(P＜0.05);术后第14天与术后第28天差异无统计学意义(P=0.473).移植组、对照组平均ADC值与ALT、AST值呈负相关(P＜0.05),与ALB值呈正相关(P＜0.05).结论 骨髓单个核细胞移植可促进急性肝损伤模型的肝功能恢复;观察肝脏平均ADC值动态变化可了解骨髓单个核细胞移植治疗急性肝损伤的疗效.

Abstract：

Objective To investigate the therapeutic effect of mononuclear bone marrow cells (MBMCs) transplantation to rabbit liver with acute hepatic injury ( AHI ) and the feasibility that evaluated the functional recovery of acute hepatic injury model with MR-DWI. Methods 10 healthy rabbits were chosen to be normal control group which was only carried out MR-DWI scan of normal liver. 42 model rabbits of acute liver injury were randomly selected and divided into 2 groups: transplanted group( n = 21 ) and control group( n =21 ). Each rabbit of the transplanted group was infused 5 ml MBMCs suspension (about containing 2 × 107 MBMCs) into its liver at multiple sites. All manipulations to each rabbit in the control group were as same as those in the transplanted group except that 5 ml of D-Hanks solution was injected instead of MBMCs suspension. 7 model rabbits respectively chosen from the transplanted group and the control group were carried out MR-DWI scan and calculated the mean ADC value of the injury liver and then were killed on day 7, day 14 and day 28 of AHI establishment after transplantation. Other comparative assays were performed including: functional assay of liver, pathological examination of liver sections. Results Before MBMCs transplantation, the difference of liver function was not significant between the transplanted group and the control group. But after MBMCs transplantation , the liver functions of the transplanted group were significantly better than those of the control group at all time points tested ( P ＜ 0. 05 ). On day 7 of AHI establishment after MBMCs transplantation, the mean ADC values of the transplanted group and the control group were significant lower than those of the normal control group(P ＜0. 05 ). The mean ADC values of the transplanted group and the control group increased to the mean ADC values of the normal control group over time. At the same time point , the mean ADC values of the transplanted group were significantly higher than those of the control group. In the transplanted group, the difference of average ADC values between any two time points were significantly (P ＜ 0. 05). In the control group, the mean ADC values on day 7 were lower than those on day 14 and day 28 (P＜0. 05), the mean ADC values on day 14 were lower than those on day 28(P =0. 417). The correlation between the average ADC value and the ALT or AST was negative( P ＜0. 05 ), the correlation between the average ADC value and the ALB was positive( P ＜ 0. 05). Along with the increase of the average ADC value, the liver function of the AHI model rabbit gradually got better. Conclusion Transplantation of MBMCs promoted the recovery of liver function of AHI model rabbit The recovery of the injury liver could be detected with observing dynamic change of its mean ADC value.

The experimental study of magnetic resonance diffusion-weighted imaging to the treatment of acute hepatic injury with allogeneic MBMCs transplantation into liver

Objective To investigate the therapeutic effect of mononuclear bone marrow cells (MBMCs) transplantation to rabbit liver with acute hepatic injury ( AHI ) and the feasibility that evaluated the functional recovery of acute hepatic injury model with MR-DWI. Methods 10 healthy rabbits were chosen to be normal control group which was only carried out MR-DWI scan of normal liver. 42 model rabbits of acute liver injury were randomly selected and divided into 2 groups: transplanted group( n = 21 ) and control group( n =21 ). Each rabbit of the transplanted group was infused 5 ml MBMCs suspension (about containing 2 × 107 MBMCs) into its liver at multiple sites. All manipulations to each rabbit in the control group were as same as those in the transplanted group except that 5 ml of D-Hanks solution was injected instead of MBMCs suspension. 7 model rabbits respectively chosen from the transplanted group and the control group were carried out MR-DWI scan and calculated the mean ADC value of the injury liver and then were killed on day 7, day 14 and day 28 of AHI establishment after transplantation. Other comparative assays were performed including: functional assay of liver, pathological examination of liver sections. Results Before MBMCs transplantation, the difference of liver function was not significant between the transplanted group and the control group. But after MBMCs transplantation , the liver functions of the transplanted group were significantly better than those of the control group at all time points tested ( P ＜ 0. 05 ). On day 7 of AHI establishment after MBMCs transplantation, the mean ADC values of the transplanted group and the control group were significant lower than those of the normal control group(P ＜0. 05 ). The mean ADC values of the transplanted group and the control group increased to the mean ADC values of the normal control group over time. At the same time point , the mean ADC values of the transplanted group were significantly higher than those of the control group. In the transplanted group, the difference of average ADC values between any two time points were significantly (P ＜ 0. 05). In the control group, the mean ADC values on day 7 were lower than those on day 14 and day 28 (P＜0. 05), the mean ADC values on day 14 were lower than those on day 28(P =0. 417). The correlation between the average ADC value and the ALT or AST was negative( P ＜0. 05 ), the correlation between the average ADC value and the ALB was positive( P ＜ 0. 05). Along with the increase of the average ADC value, the liver function of the AHI model rabbit gradually got better. Conclusion Transplantation of MBMCs promoted the recovery of liver function of AHI model rabbit The recovery of the injury liver could be detected with observing dynamic change of its mean ADC value.