癌基因CTTN编码蛋白Cortactin与大肠癌细胞内吞作用的关系

目的 分析Cortactin分子与大肠癌细胞内吞作用的关系,了解癌组织高表达Cortactin的内在意义.方法 采用免疫组化和蛋白印迹检测大肠癌组织、癌旁组织及大肠癌细胞系中Cortactin表达水平;采用小干扰RNA抑制癌细胞Cortactin表达,并构建Cortactin不同功能区缺失突变体,检测各种癌细胞Tfn内...

A pivotal role of cortactin, a CTTN encoding protein, in endocytosis of human colon cancer

Objective To explore the role of cortactin in endocytosis of colon cancer cells and clarify the significance of its over-expression in colon cancer tissues.Methods Immunohistochemistry and Western blot were employed to detect the expression of cortactin in benign and malignant tissues and cells.Cell endocytosis was examined in cancer cells after siRNA treatment and DNA transfection with plasmid encoding cortactin wild type and domain deletion mutants.Results Cortactin was over-expressed in colon cancer tissues than in adjacent normal tissues.The expression rate was 77.5% in cancer tissues and 47.5% in normal tissues ( P ＜ 0.05 ).The value of transferrin uptake was 0.61 ± 0.02 in siRNA treated cancer cells and 1.01 ± 0.16 in the control cells (P ＜ 0.05).Intact molecule and sufficient level of cortactin was required for an optimal endocytosis of cancer cells.Cortactin was involved in coated-vesicle transportation in cells.Conclusion Endocytosis in colon cancer cells is dependent on an intact expression of CTTN.An over-expression of cortactin facilitates the signaling in invasion and metastasis related to endocytosis.