牛奶和辅酶Q10对丙烯腈致血管内皮功能紊乱的影响

目的 探讨牛奶或辅酶Q10预处理对丙烯腈致大鼠血管内皮功能紊乱的影响.方法 将80只大鼠分为4组:对照组、单纯丙烯腈组、牛奶组、辅酶Q10组.采用灌胃染毒法,对照组仅予玉米油(丙烯腈的溶剂,1 ml/100 g),其他3组予丙烯腈25 mg/kg染毒.牛奶组和辅酶Q10组在染毒前30 min分别给予牛奶、辅酶Q10预处理.染毒12周后检测血清及主动脉组织中诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)的活力.结果 单纯丙烯腈组、牛奶组、辅酶Q10组血清iNOS水平(42.9±2.5)U/ml、(26.5±4.4)U/ml、(26.7±3.3)U/ml]比对照组(21.9±1.6)U/ml,P＜0.05]升高.主动脉组织中iNOS在单纯丙烯腈组、牛奶组、辅酶Q10组(0.812±0.008)、(0.773±0.019)、(0.622±0.013)U/mg蛋白]比对照组(0.540±0.028)U/mg蛋白,P＜0.05]高;而辅酶Q10组的主动脉eNOS活力(0.471±0.011)U/mg蛋白]高于对照组、单纯丙烯腈组和牛奶组(0.371±0.029)、(0.380±0.016)、(0.425±0.020)U/mg蛋白,P＜0.05].结论 牛奶和辅酶Ql0可以缓解丙烯腈致血管内皮功能紊乱作用.

Abstract：

Objective To explore the influences of milk or coenzyme Q10 pretreatment to acrylonitrile on vascular endothelial functions in rats.Methods A total of 80 rats were randomly divided into 4 groups:control group(Con),acrylonitrile exposure group(ACN),milk pretreatment group (M + ACN)and coenzyme Q10 pretreatment group(Q10 + ACN).The experiment was conducted by the method of gavage exposure in rats.Control group was exposed to corn oil;acrylonitrile was administered to other three groups at the doses of 25 mg/kg.The M + ACN and Q10 + ACN groups were pretreated by milk or coenzyme Q10 at 30 minutes before acrylonitrile exposureAfter a 12-week exposure,the activities of inducible nitric oxide synthase(iNOS)and endothelial nitric oxide synthase(eNOS)were measured in serum and aortal tissues.Results As compared with Con group(21.9 ± 1.6)U/ml],the activity of blood serum iNOS was higher in ACN,M + ACN and Q10 + ACN groups(42.9 ± 2.5)U/ml,(26.5 ± 4.4)U/ml,(26.7 ±3.3)U/ml,P＜0.05].As compared with Con group(0.540 ± 0.028)U/mgprot],the activity of aortal iNOS was higher in ACN,M + ACN and Q10 + ACN groups(0.812 ± 0.008),(0.773 ± 0.019),(0.622 ±0.013)U/mgprot,(P ＜0.05)].Furthermore the activity of aortal eNOS in Q10 + ACN group(0.471 ±0.011)U/mgprot]was higher than Con,ACN or M +ACN group(0.371 ±0.029),(0.380 ±0.016),(0.425 ±0.020)U/mgprot,P ＜0.05].Conclusion Chronic administration of ACN by gavages results in vascular endothelial dysfunctions.Milk and coenzyme Q1o pretreatment reduce this effect in rats.

Effects of milk and coenzyme Q10 on the interference of acrylonitrile on vascular endothelial functions

Objective To explore the influences of milk or coenzyme Q10 pretreatment to acrylonitrile on vascular endothelial functions in rats.Methods A total of 80 rats were randomly divided into 4 groups:control group(Con),acrylonitrile exposure group(ACN),milk pretreatment group (M + ACN)and coenzyme Q10 pretreatment group(Q10 + ACN).The experiment was conducted by the method of gavage exposure in rats.Control group was exposed to corn oil;acrylonitrile was administered to other three groups at the doses of 25 mg/kg.The M + ACN and Q10 + ACN groups were pretreated by milk or coenzyme Q10 at 30 minutes before acrylonitrile exposureAfter a 12-week exposure,the activities of inducible nitric oxide synthase(iNOS)and endothelial nitric oxide synthase(eNOS)were measured in serum and aortal tissues.Results As compared with Con group(21.9 ± 1.6)U/ml],the activity of blood serum iNOS was higher in ACN,M + ACN and Q10 + ACN groups(42.9 ± 2.5)U/ml,(26.5 ± 4.4)U/ml,(26.7 ±3.3)U/ml,P＜0.05].As compared with Con group(0.540 ± 0.028)U/mgprot],the activity of aortal iNOS was higher in ACN,M + ACN and Q10 + ACN groups(0.812 ± 0.008),(0.773 ± 0.019),(0.622 ±0.013)U/mgprot,(P ＜0.05)].Furthermore the activity of aortal eNOS in Q10 + ACN group(0.471 ±0.011)U/mgprot]was higher than Con,ACN or M +ACN group(0.371 ±0.029),(0.380 ±0.016),(0.425 ±0.020)U/mgprot,P ＜0.05].Conclusion Chronic administration of ACN by gavages results in vascular endothelial dysfunctions.Milk and coenzyme Q1o pretreatment reduce this effect in rats.