RP-HPLC测定头孢克肟的含量

目的建立测定头孢克肟含量的反相高效液相色谱法。方法采用HypersilBDSC18（4．6mm×250mm，10μm）色谱柱，流动相为0．1mol·L^-1醋酸铵缓冲液（用氨试液调pH为7．0）-乙腈（96：4），流速1．0mL．min^-1，检测波长254nm,柱温35℃。结果头孢克肟浓度在102．38～1433．45μg·mL^-1内，峰面积与浓度呈良好线性关系（r=0．9999）；头孢克肟的最低检测限为0．18ng，最低定量限为0．60ng，含量测定结果与按BP2008年版法定标准方法测定结果没有显著性差异。结论本法简便、专属性强，可用于头孢克肟含量的测定。

Determination of Cefixime by RP-HPLC

OBJECTIVE To establish a method for the determination of cefixime by RP-HPLC. METHODS Cefixime was separated on a Hypersil BDS C18 column （4.6 mm×250 mm, 10μm） with a mixture of 0.1 mol·L^-1 ammonium acetate （adjusted with ammonia solution to pH 7.0） and acetonitrile （96 ：4） as mobile phase. The flow rate was 1.0 mL·min^-1. The detection wavelength was 254 nm. The column temperature was 35℃. RESULTS The calibration curve was linear within the range of 102.38-1 433.45 μg.mL^-1 for cefixime （r=0.999 9）. The limit of detection and limit of quantitation were 0.18 ng and 0.60 ng respectively. Statistical analysis by t-test showed no significant differences between the results obtained by the method and by BP 2008. CONCLUSION This method is simple and selective for the determination of cefixime.