99mTc标记抗卵巢癌单克隆抗体放射免疫导向手术的实验研究

进行＾９９ｍＴｃ标记卵巢癌单抗放射免疫导向手术研究，为临床应用提供基础。方法：彩用改进预锡法标记ＣＯＣ１８２Ｂ２－Ｆ（ａｂ’）２（单抗组）和＾９９ｍＴｃ－ＭＭＩｇＧ（对照组）。首先在体外进行放射免疫显像（ＲＩＩ）初步决定探测时机，然后采用探讨测仪（ＧＤＰ）首先在外体进行放射免疫显及临年正常组织放射性测量，并计算肿瘤与非肿瘤放射性比值（Ｔ／ＮＴ）。．结果１改进预锡法单抗＾９９ｍＴｃ标记率达计算肿瘤与

Experimental study of radioimmunoguided surgery with99mTc labeling monoclonal antibody in ovarian carcinoma

Objective:To study radioimmunoguided surgery (RIGS) system with99mTc labeling monoclonal antibody (McAb) for detection of ovarian carcinoma in nude mice bearing human ovarian carcinoma.Methods:McAb COC183 B2-F(ab′)2 and normal mice IgG labelled with99mTc were prepared according to improved pretining method and were injected respectively into two groups of nude mice bearing human ovarian carcinoma with ascites.Radioimmunoimaging(RII) were performed before operations to determine the correct time of operation.The radioactivity of tumor and their neighboring tissues were measured by GDP in vivo and compared with gamma well counts after dissection in vitro.Results:COC183B2-F(ab′)2 labelled with99mTc yielded a labeling rate of over 95% and a good immunoactivity.The detection of carcinoma tissue by GDP showed T/NT(liver) 3.27±0.64，T/NT(spleen) 2.23±0.47,T/NT(intestine)0.33±0.84,T/NT(muscle)4.68±0.81 and T/NT ratio of 2:1 was diagnosed positive for cancer.The smallest carcinoma detected was 0.3cm×0.1cm and the nearest distance between carcinoma and normal tissue was 0.2cm.The results by RII and GDP showed that the operation should be performed in 18 hours after injecting99mTc labelled COC183B2-F(ab′)2.Conclusions:The above studies proved that RIGS with99mTc labelled COC183B2-F(ab′)2 is practical and hopefully be used clinically for ovarian carcinoma.