荧光定量聚合酶链反应对生殖器疱疹病毒感染的检测

目的 探讨荧光定量聚合酶链反应（FQ-PCR）在诊断生殖器疱疹病毒感染中的应用。方法 本研究共分两组，检测组即就诊的疑似生殖器疱疹患者347例，对照组12例。分别用FQ-PCR方法检测两组送检标本中的单纯疱疹病毒（HSV）DNA。结果 347例疑似生殖器疱疹患者送检标本中，HSVDNA阳性139例，阳性率为40．1％（139／347），其中阳性标本中有皮损组织液75例、男性尿道拭子31例、女性宫颈分泌物33例，各类标本的阳性率依次为91．5％（75／82）、21．1％（31／147）、28．0％（33／118）。HSVDNA阳性人群中性别差异无统计学意义（P〉O．05，X^2检验）。12例对照标本中没有检出HSVDNA。结论 分泌物中检出的HSVDNA能直观地反映患者当前病毒感染状况，在有皮损组织的患者中，HSVDNA的检出率更高，FQ-PCR能准确、快速地对生殖器疱疹病毒感染做出诊断。

Clinical application of fluorescent quantitative PCR in detection of genital herpesvirus infection

Objective TO explore the clinical application of fluorescence quantitative polymerase chain reaction （FQ-PCR） in detection of genital herpesvirus infection. Methods The study was performed on 347 doubtful genital herpes cases （experimental group） and 12 cases of healthy controls （control group）. FQ-POR was applied to detecting herpes simplex-deoxyribonucleic acid （HSV-DNA） in tissue fluids such as vesicle fluids and genital tract swabs. Results In 347 specimens from patients who were suspected to be infected with HSV,there were 139 cases in which H SV-DNA was detected,and the total positive rate was 40.1% （139/347）. Among the 139 PCR-positive specimens, there were 75 cases of tissue fluids, 31 cases of urethral swab and 33 cases of cervix secretions,and the positive rate was 91.5%（75/82）, 21.1%（31/147）, 28.0%（33/118） respectively. The positive rates were no significantly different Via ;（2 test （P〉0.05） between the males and the females. And HSV-DNA wasn＇t detected in the 12 specimens of negative controls. Conclusion The detected HSV-DNA can directly reflect the current infection status of patients, especially for patients with skin lesion, the detection rate of HSV-DNA is more higher. FQ-PCR may be a very good tool in diagnosis of genital herpesvirus infection correctly and rapidly.