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雷公藤内酯醇对皮肤鳞状细胞癌A431细胞株增殖与凋亡的影响
引用本文:陈明亮,谭帅,张桂英,易梅,简丹,谢红付,陈翔. 雷公藤内酯醇对皮肤鳞状细胞癌A431细胞株增殖与凋亡的影响[J]. 中南大学学报(医学版), 2009, 34(7): 638-641
作者姓名:陈明亮  谭帅  张桂英  易梅  简丹  谢红付  陈翔
作者单位:中南大学,湘雅医院皮肤科,长沙,410008;中南大学,湘雅二医院皮肤科,长沙,410011
基金项目:湖南省卫生厅科研项目 
摘    要:目的:探讨雷公藤内酯醇(triptolide,TP)对体外培养的人皮肤鳞状细胞癌A431细胞株增殖与凋亡的影响.方法:以体外培养的人皮肤鳞状细胞癌A431细胞株为研究对象,MTT比色法测定TP对A431细胞株增殖活性的影响,光镜观察细胞形态学的改变,流式细胞仪检测TP作用于A431细胞株后细胞周期的分布及凋亡率的变化.结果:随着TP剂量的增加及作用时间的延长,TP对A431细胞株增殖活性的抑制作用增强,TP不但能够引起细胞形态的改变(漂浮细胞及核固缩现象增多),而且能够诱导A431细胞株凋亡,改变细胞周期的分布,与对照组相比G0/G1期细胞比例增多(P<0.05),S期细胞比例减少(P<0.05).结论:TP通过诱导A431细胞凋亡并抑制其增殖发挥抗肿瘤作用.

关 键 词:雷公藤内酯醇  鳞状细胞癌  增殖  细胞周期  凋亡
收稿时间:2009-02-04

Effect of triptolide on the proliferation and apoptosis ofhuman epidermal squamous cell carcinoma cell line A431 in vitro
CHEN Mingliang,TAN Shuai,ZHANG Guiying,YI Mei,JIAN Dan,XIE Hongfu,CHEN Xiang. Effect of triptolide on the proliferation and apoptosis ofhuman epidermal squamous cell carcinoma cell line A431 in vitro[J]. Journal of Central South University. Medical sciences, 2009, 34(7): 638-641
Authors:CHEN Mingliang  TAN Shuai  ZHANG Guiying  YI Mei  JIAN Dan  XIE Hongfu  CHEN Xiang
Affiliation:1.Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008;
2.Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha 410011, China
Abstract:Objective To investigate the effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro. Methods Human epidermal squamous cell carcinoma cell line A431 was cultured. After the treatment with triptolide, the inhibi-tion of cellular growth was determined by measuring MTT dye absorption of the living cells. Light mi-croscope showed morphological changes. The cell cycle and apoptosis rate were assessed by flow cy-tometry. Results Triptolide could significantly inhibit the proliferation of A431 cells in a dose- and time-dependent manner. Triptolide could also cause cell morphological changes ( the number of float-ing cells and nuclear pyknosis increase), induce cell apoptosis, and change the distribution of cell cycle phase in A431 cells. Compared with the control group, the G0/G1 phase A431 cell rate in-creased and the rate of S phase cell decreased in TP-treated group. Cell cycles were obviously inhibi-ted by triptolide in G0/G1 phase (both P<0.05). Conclusion TP could play an anti-tumor role by effectively inducing cell apoptosis and inhibiting the proliferation of A431 cells.
Keywords:triptolide  carcinoma,squamous cell  proliferation  cell cycle  apoptosis
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