Assessment of tick-borne pathogens presence in Dermacentor reticulatus ticks in north-eastern Poland |
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| Affiliation: | 1. Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, Bialystok, Poland;2. Department of Allergy, Guy’s and St Thomas’ Hospital NHS Foundation Trust, London, United Kingdom;1. Department of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, Bialystok, Poland;2. Department of Physiology, Medical University of Bialystok, Bialystok, Poland;3. Department of Radiology, University Children’s Clinical Hospital of Bialystok, Bialystok, Poland;1. Reconstructive Orthopaedic Surgery and Innovative Techniques – Musculoskeletal Tissue Bank, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy;2. BioEngLab, Health Science and Technology – Interdepartmental Center for Industrial Research (HST-CIRI), University of Bologna, Cesena, Italy;3. Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy;4. Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna, Italy;5. Laboratory of Cellular and Molecular Engineering “Silvio Cavalcanti” - DEI, University of Bologna, Cesena, Italy;1. Department of Ophthalmology, University of Lübeck, Lübeck, Germany;2. Laboratory for Angiogenesis & Ocular Cell Transplantation, University of Lübeck, Lübeck, Germany;1. Federal State Public Science Institution, Scientific Centre of Family Health and Human Reproduction Problems, Irkutsk, Russia;2. Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan;3. Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan;4. United Graduate School of Agricultural Science & Veterinary Science, Gifu University, Gifu, Japan;5. School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan;6. Faculty of Pharmaceutical Sciences, Chiba Institute of Science (CIS), Choshi, Japan;1. Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Przybyszewskiego Str. 63/77, 51-148, Poland;2. Department of Geoinformatics and Cartography, Institute of Geography and Regional Development, University of Wroclaw, Wroclaw, Pl. Uniwersytecki 1, 50-137, Poland |
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| Abstract: | PurposeDermacentor reticulatus is the second most common tick species in Poland after Ixodes ricinus. The aim of the study was to analyze the presence of pathogen DNA in D. reticulatus.Materials and methodsTicks were collected in The Protected Landscape Area of the Bug and Nurzec Valley (52°40′ N and 22°28’ E) between 2016 and 2017. End-point PCR for Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Babesia spp., Rickettsia spp., Bartonella spp. and Coxiella burnetii detection was performed.ResultsTick-borne pathogens’ DNA was detected in 11.3% of 301 ticks: B. burgdorferi s.l. in 3.6%, Babesia spp. in 6.3%, A. phagocytophilum in 0.7% and B. burgdorferi s.l.-Babesia spp. co-infection in 0.7%. In all 21 Babesia spp. positive samples, sequence analysis confirmed the presence of Babesia canis with an 80.3%–98.3% homology with the B. canis sequences in GenBank. C. burnetii, Bartonella spp., and Rickettsia spp. DNA were not detected.ConclusionsDermacentor reticulatus from north-eastern Poland were found to carry three of the most common tick-borne pathogens (B. burgdorferi s.l., Babesia canis, A. phagocytophilum) which lead to single and mixed infections. Babesia canis was the most prevalent pathogen identified in D. reticulatus. |
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