Comparison of MALDI-TOF MS,gene sequencing and the Vitek 2 for identification of seventy-three clinical isolates of enteropathogens |
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Authors: | Jiankai Deng Liang Fu Ruilian Wang Nan Yu Xixia Ding Lingxiao Jiang Yanping Fang Changhong Jiang Lijuan Lin Ying Wang Xiaoyan Che |
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Affiliation: | Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China |
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Abstract: | ObjectiveThis study was performed to evaluate the analytical and practical performance of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) compared to the sequencing method and the Vitek 2 system for identification of enteropathogens in the clinical microbiology laboratory.MethodsTen type strains and 73 clinical isolates of enteropathogens representing eight genera were analyzed by MALDI-TOF MS. All isolates were also characterized by gene sequencing allowing interpretation of the results from MALDI-TOF MS. In addition, MALDI-TOF MS was compared with the Vitek 2 system for the identification of ten isolates of Aeromonas and six of Salmonella.ResultsAs previously known, identification between Shigella and Escherichia coli is not possible to distinguish. MALDI-TOF MS produced the correct identifications for all other type strains and clinical isolates to the genus level. Fifteen Campylobacter jejuni, six Campylobacter coli, three Plesiomonas shigelloides, three Yersinia enterocolitica, two Clostridium difficile, one Vibrio parahaemolyticus, one Vibrio fluvialis, and one Vibrio cholera were all correctly identified to the species level. Genus and species identifications of ten Aeromonas and six Salmonella isolates by MALDI-TOF MS were consistent with those by the Vitek 2, but with much less cost and about ten times faster.ConclusionsThis study demonstrates that MALDI-TOF MS is a powerful tool for fast, accurate and low-cost identification of enteropathogens in the clinical microbiology laboratory. |
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Keywords: | Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) enteropathogens microbial identification clinical microbiology |
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