Opto-electronic DNA chip-based integrated card for clinical diagnostics |
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Authors: | Gilles Marchand Patrick Broyer Véronique Lanet Cyril Delattre Frédéric Foucault Lionel Menou Bernard Calvas Denis Roller Frédéric Ginot Raymond Campagnolo Frédéric Mallard |
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Institution: | (1) Equipe commune bioMérieux/Leti, CEA/Leti/DTBS, 17 avenue des Martyrs, 38054 Grenoble Cedex 09, France;(2) Department of Technology for Biology and Health, CEA/Leti, 17 avenue des Martyrs, 38054 Grenoble Cedex 09, France;(3) bioMérieux SA, Chemin de l’Orme, 69280 Marcy L’Etoile, France;(4) Smart Packaging Solutions, Avenue Olivier PERROY, Zone Industrielle de ROUSSET, 13106 ROUSSET cedex, France;(5) SNPE Matériaux Energétiques, Centre de Recherches du Bouchet, Rue Lavoisier, 91710 Vert le Petit, France |
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Abstract: | Clinical diagnostics is one of the most promising applications for microfluidic lab-on-a-chip or lab-on-card systems. DNA
chips, which provide multiparametric data, are privileged tools for genomic analysis. However, automation of molecular biology
protocol and use of these DNA chips in fully integrated systems remains a great challenge. Simplicity of chip and/or card/instrument
interfaces is amongst the most critical issues to be addressed. Indeed, current detection systems for DNA chip reading are
often complex, expensive, bulky and even limited in terms of sensitivity or accuracy. Furthermore, for liquid handling in
the lab-on-cards, many devices use complex and bulky systems, either to directly manipulate fluids, or to ensure pneumatic
or mechanical control of integrated valves. All these drawbacks prevent or limit the use of DNA-chip-based integrated systems,
for point-of-care testing or as a routine diagnostics tool. We present here a DNA-chip-based protocol integration on a plastic
card for clinical diagnostics applications including: (1) an opto-electronic DNA-chip, (2) fluid handling using electrically
activated embedded pyrotechnic microvalves with closing/opening functions. We demonstrate both fluidic and electric packaging
of the optoelectronic DNA chip without major alteration of its electronical and biological functionalities, and fluid control
using novel electrically activable pyrotechnic microvalves. Finally, we suggest a complete design of a card dedicated to automation
of a complex biological protocol with a fully electrical fluid handling and DNA chip reading. |
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Keywords: | Opto-electronic detection Pyrotechnic microvalves Clinical diagnostics |
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