fascin、nm23和C—erbB-2蛋白在胃癌中的表达及临床意义

目的探讨胃癌组织中fascin、nm23和C—erbB-2蛋白的表达情况及在胃癌发生、发展及转移中的意义，为临床诊断、治疗及判断预后提供思路。方法用免疫组织化学方法检测76例胃癌组织，自身对照正常胃壁组织及其中53例淋巴结转移肿瘤组织中fascin表达，以及胃癌组织中nm23、C—erbB-2的表达。实验所获数据进行统计学分析。结果正常胃壁组织无fascin表达，76例胃癌中有24例（31．6％）fascin表达阳性，39例（51-3％）nm23表达阳性，35例（46．1％）C—erbB-2表达阳性，fascin表达与胃癌病理类型（WHO分类、Lauren’s分型）、TNM分期、浆膜侵犯、淋巴结转移、远处转移、nm23表达及C—erbB-2表达等因素相关，与年龄、性别、脉管癌栓等因素无关。在伴有转移的胃癌病例中，原发灶和转移淋巴结中fascin蛋白表达有相关性。结论fascin蛋白的表达与胃癌发生、局部发展和转移相关，并在胃癌及其转移灶中有表达连续性；在胃癌组织中fascin表达与nm23表达呈负相关、与C—erbB-2表达呈正相关，检测三种基因表达有助于胃癌侵袭转移的判断，fascin有可能成为新的治疗靶点。

Expression of fascin, nm23 and C-erbB-2 in gastric carcinoma and its clinical significance

Objective To investigate the clinical significance of fascin, nm23 and C-erbB-2 expression in the carcinogenesis, development and metastasis of gastric carcinoma, providing a new way for clinical diagnosis, treatment and judging prognosis. Methods The expression of fascin in 76 tissues of gastric carcinoma, their own normal gastric tissues in contrast, and the lymph nodes involved by gastric carcinoma in 53 cases were detected with immunohistochemical stain. The expression of nm23 and C-erbB-2 in the tissues of gastric carcinoma were also detected. The data from the experiment were analyzed statistically. Results There was no expression of fascin in normal gastric tissues. In the 76 gastric carcinoma, fasein proteins were positive in 24 cases（31.6%）, nm23 in 39 cases（51.3 % ）, C-erbB-2 in 35 ca.ses（46.1% ）. The expression of fascin protein correlated with pathological style （WHO classification and Lauren＇s typing）, TNM staging, serosal invasion, lymphatic metastasis, distant metastasis, the expression of nm23 and C-erbB-2, and so on. It didn＇t correlate with age, gender, cancer embolus in vessel and so on. In the cases with metastasis, the expressions of fascin in primary masses and their metastatic lymph nodes were correlative. Conclusions the expression offascin usually correlates with the occurrence, development and metastasis of gastric carcinoma. The expression of fascin in gastric carcinoma and its metastasis are continuous. In the gastric carcinoma, the expression offascin is positively correlative with the expression ofC-erbB-2, but negatively correlative with the expression of nm23. The expression ofthe three genes can help to assess the invasion and metastasis of gastric carcinoma. Fascin may be a new therapeutic target of gatric carcinoma