高效液相色谱法测定复方替硝唑醇溶液中替硝唑氯霉素的含量

目的探讨同时测定复方替硝唑醇溶液中替硝唑和氯霉素含量的方法。方法采用高效液相色谱法，色谱柱为DiamonsilC18（250mm×4．6mm，5μm），流动相为甲醇-水（50：50），检测波长为290nm。结果替硝唑、氯霉素在20～120μg/ml浓度范围内均有良好的线性关系，相关系数均为0．9999，平均回收率分别为100．62％、100．54％，相对标准差分别为0．67％、0．52％。结论本方法分离度好、快速、简便，可用于含替硝唑、氯霉素的质量控制。

Simultaneous determination of tinidazole and chloramphenirol in compound tinidazole tincture by HPLC

Objective To establish a HPLC method for simultaneous determination of tinidazole anol ehloramphenieol in compound tinidazole tincture. Methods The method included a Diamonsil C18 column （250 mm× 4.6 mm,5 μ m）and methanol -water（50： 50） as the mobile phase ane an ultraviolet detecting wavelength of 290 nm. Results The calibration curve was linear in a concentration range of （20 - 120） μg/ml of both tinidazole and ehloramphenieol（ r =0. 9999）. The average recoveries of tinidazole and ehloramphenieol were 100. 62%, 100. 54% and RSD were 0.67% ,0. 52% respectively.Conclusion The method for the determination of tinidozole and chloramphenicol was rapid and convenient. It can be used for the quality control of tinidazole and chloramphenicol.