| Abstract: | Objective To study the apoptosis of cultured rabbit smooth muscle cells (SMCs) by low dose 32Pperfused in balloon catheter and its doseresponse relationship differences. Methods Liquid 32P isotope wasinfused directly into the balloon of PTCA catheter, to irradiate the SMCs with various doses. Light microscope,electron microscope, agarose gel electrophoreses and flow Cytometry(FCM) were used to detect the apoptosis ofSMCs after 24h irradiation. Results The irradiated SMCs showed condensation of the cytoplasm, chromatincondensation, chromosal clumping and margination, apoptotic body and the nuclear DNA degradation on agarose,the amount of SMC opoptosis was positively correlated with the different doses in FCM. Conclusion Our dataidentified irradiation with 32P isotopefilled balloon can be the induction of SMCs apoptosis. Further study isrequired to determine potential value of this method about prevention of restenosis resulting from decreasing ofSMCs proliferation. |
