Predicted structure of the adenovirus DNA binding protein |
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Authors: | Joseph M. Weber Faxing Cai Joseph Horvath J. Guy Guillemette |
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Affiliation: | (1) Department of Microbiology, Faculty of Medicine, Université de Sherbrooke, J1H 5N4 Sherbrooke, Québec, Canada;(2) Present address: St. Joseph's Cancer Institute, 3001 W. Dr. Martin Luther King Jr. Blvd., P.O. Box 4227, 33677-4227 Tampa, FL |
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Abstract: | The DNA sequence of a portion of the MAV1SmaI-D fragment coding for the C-terminal 147 amino acids of the adenoviral DNA-binding protein (DBP) has been determined. A multiple sequence alignment was constructed of the MAV1 fragment and the DBPs of Ad.2, 4, 5, 7, 12, 40, and 41 to examine the degree of conservation of features that have been mapped on the Ad.2 DBP and to identify further conserved features. The less conserved N-terminal segment of the protein contains two nuclear localization signals and two acidic regions, the host range region, and all of the 11 phosphorylation sites. The highly conserved C-terminal segment contains a potential leucine zipper and zinc finger motifs. These sequence features were mapped onto a predicted secondary structure of the Ad.2 DBP.The nucleotide sequence data used in translation in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M81056 |
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Keywords: | adenovirus, murine DNA binding protein, adenovirus DNA replication, proteins zinc finger leucine zipper |
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