SLE患者可溶性抗原及自身抗体检测与临床意义研究

目的：探讨ＳＬＥ患者免疫发病机理及其临床意义。方法：免疫印迹技术测定ＥＮＡ抗体，免疫金标法测定ｄｓ－ＤＮＡ抗体，ＥＬＩＳＡ法测ＡＮＡ、ｓＣＤ１０６、ｓＣＤ９５、ｓＣＤ２５、ｓＣＤ５４。ＥＳＲ按临床检测常规进行。结果：活动性ＳＬＥ患者ｓＣＤ１０６、ｓＣＤ５４、ｓＣＤ２５水平均高于非活动性ＳＬＥ患者的相应值（Ｐ〈０．０５）；而后者又分别高于正常对照组的相应值（Ｐ〈０．０５）；活动期ＳＬＥ患者ｓＣＤ９５水平高于非活动期或正常对照组，后两者ｓＣＤ９５水平无差别（Ｐ〉０．０５）。相关分析表明，ｓＣＤ１０６和ｓＣＤ５３相关（ｒ＝０．５４９，Ｐ〈０．０５），ｓＣＤ９５与ｓＣＤ２５正相关（ｒ＝０．５６８，Ｐ〈０．０５），ＥＳＲ与ｓＣＤ１０６、ｓＣＤ５４、ｓＣＤ９５及ｓＣＤ２５均相关，ＡＮＡ只与ｓＣＤ９５相关，ｄｓ－ＤＮＡ在活动

Study on soluble antigen and autoantibodies in Patients with SLE

Objective:To detect the immunological mechanism and its value. Methods:ELISA method was used to detect ANA, sCD106,sCD95,sCD25 and sCD54. ENA was detected by immuno blot, ds DNA was measured by immuno gold technique, ESR was detected by clinical regular method.Results:Serum sCD106,sCD54 or sCD25 or SLE patients in active period, and the three marks in non active period, and the three marks in non active period was higher than that of the normal controls. sCD95 of SLE patients in active period was higher than that of noraml controls or of SLE petients in non active period. sCD106 was relatedto sCD54 obviously (r=0.549,P<0.05), sCD95 was related to sCD25 (r=0.568,P< 0.05 ).ESR was related to sCD106,sCD54,sCD95 and sCD25 (P< 0.05 ). ANA was related to sCD95 only. Difference of positive percent of ds DNA was obvious between active SLE patients and nonactive patients. Conclusion :Immunological status in SLE patients was abnormal. Serum sCD54,sCD95,sCD106 or sCD25 could be used as the mark of SLE activity.