顺铂对豚鼠耳蜗形态与Bcl-2表达的影响

目的 探讨顺铂对豚鼠耳蜗毛细胞耳毒性作用的形态学改变以及顺铂对螺旋神经节细胞Bcl-2蛋白表达的影响.方法 在实验动物被处死前,检测其畸变产物耳声发射（DPOAE）幅值变化.取左侧耳蜗,行耳蜗铺片,观察基底膜毛细胞.右侧耳蜗冰冻切片,用免疫组织化学方法检测Bcl-2的表达.结果 对照组耳蜗铺片显示内、外毛细胞结构完整,排列整齐.顺铂组毛细胞肿胀,纤毛缺失,DPOAE幅值和螺旋种经节的Bcl-2表达明显低于正常对照组（P＜0.01）.结论 顺铂引起耳蜗内、外毛细胞发生形态学改变,其耳毒性作用可能与抑制Bcl-2蛋白的表达有关.

Effect of Cisplatin on Morphology and Expression of Bcl-2 Protein in Guinea Pig Cochlea

Objective To study the effect of ototoxicity induced by cisplatin on morphology of haircells and expression of Bcl-2 protein in spiral ganglion cells.Methods The amplitudes of distortion product otoacoustic emission(DPOAE) were measured before all the animals were killed. Left cochleas were taken for cochlear surface preparation to observe haircells in basilar membrane,right cochleas for frozen sections to examine the expressions of Bcl-2 by immunohistochemical method.Results The inner and outer haircells in saline group were kept in integrate structure and regular distribution,swollen haircells and lost cilia were observed in cisplatin group.The amplitudes of DPOAE and expression of Bcl-2 protein in spiral ganglion cells were lower in cisplatin group than in saline group(P<0.01).Conclusion Cisplatin caused the morphology damage of inner and outer haircells and downregulated the expression of Bcl-2 protein.