| 促性腺激素释放激素类似物对大鼠胃肠道5-羟色胺分泌的影响 |
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| 引用本文： | 朱晓东,王玉珍,刘晓宁,黄威权. 促性腺激素释放激素类似物对大鼠胃肠道5-羟色胺分泌的影响[J]. 解剖学报, 2003, 34(5): 518-521 |
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| 作者姓名： | 朱晓东 王玉珍 刘晓宁 黄威权 |
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| 作者单位： | 1. 中国科学技术大学生命科学院分子生物学与细胞生物学系,合肥,230027
2. 第四军医大学组织学与胚胎学教研室,西安,710033 |
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| 基金项目： | 国家自然科学基金资助项目 (39770 388) |
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| 摘 要： | 目的 研究GnRH对胃肠道内5-HT分泌的影响。方法胃腔直接注射GnRH类似物阿拉瑞林(A1arelin GnRH—A)以模拟外分泌产生的GnRH，并以免疫组织化学、高压液相色谱电化学检测(HPLC-ECD)的方法对阿拉瑞林刺激后胃及十二指肠内5-HT免疫反应细胞、血清中5-HT含量进行检测。结果 胃腔内注射GnRH—A后，大鼠胃及十二指肠内5-HT免疫反应阳性细胞密度显著增多，但血清中5-HT含量显著减少。结论 外分泌的OnRH对于5-HT的释放起明显抑制作用，但对5-HT的合成可能不产生影响。
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| 关 键 词： | 促性腺激素释放激素 大鼠 胃肠道 5-羟色胺 分泌 免疫组织化学 高压液相色谱电化学检测 |
THE EFFECT OF GnRH-A IN THE STOMACH ON THE SECRETION OF 5-HT IN THE DIGESTIVE TRACT OF RATS |
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| ZHU Xiao-dong ,WANG Yu-zhen ,LIU Xiao-ning ,HUANG Wei-quan. THE EFFECT OF GnRH-A IN THE STOMACH ON THE SECRETION OF 5-HT IN THE DIGESTIVE TRACT OF RATS[J]. Acta Anatomica Sinica, 2003, 34(5): 518-521 |
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| Authors: | ZHU Xiao-dong WANG Yu-zhen LIU Xiao-ning HUANG Wei-quan |
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| Affiliation: | ZHU Xiao-dong 1,WANG Yu-zhen 1,LIU Xiao-ning 2,HUANG Wei-quan 2* |
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| Abstract: | Objective To study the effect of GnRH on the secretion of 5-HT in the stomach. Methods The GnRH-A Alarelin was directly injected into the stomach of rats to observe the change of density of 5-HT immunoreactive positive cell in the stomach and small intestine by immunohistochemical SABC method and 5-HT level in the circulating blood by HPLC-ECD, Results Compared with the control group,which was injected with saline into the stomach,the density of immunoreactive positive cell was significantly increased in the stomach and small intestine in the GnRH-A treatment group;while 5-HT level in the circulating blood was significantly reduced in the experimental group.Conclusion GnRH can inhibit the releasing of 5-HT but not change its synthesis. |
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| Keywords: | GnRH-A 5-HT Immunohistochemical HPLC-ECD Rat |
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