| 乏氧环境下益气养精方对肺癌细胞apelin分子表达的影响＊ |
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| 引用本文： | 卢文峰,杨茜雯,董昀,崔清,蔡霄月,汪宇涵,张铭,邓海滨,徐振晔. 乏氧环境下益气养精方对肺癌细胞apelin分子表达的影响＊[J]. 世界科学技术-中医药现代化, 2019, 21(11): 2531-2538 |
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| 作者姓名： | 卢文峰 杨茜雯 董昀 崔清 蔡霄月 汪宇涵 张铭 邓海滨 徐振晔 |
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| 作者单位： | 上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海交通大学附属胸科医院中西医结合科 上海 200030,上海中医药大学附属龙华医院肿瘤科 上海 200032,上海中医药大学附属龙华医院肿瘤科 上海 200032 |
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| 摘 要： | 目的 观察益气养精方对乏氧微环境下肺癌细胞中具有促进细胞增殖侵袭及血管形成的apelin/APJ信号通路的影响。方法 CoCl2加入培养基模拟乏氧微环境,实验分组为乏氧组、常氧益气养精方组、乏氧益气养精方组及空白对照组,分别作用于人肺癌细胞株A549和H1975。采用CCK-8法和Transwell实验观察各组细胞的增殖、侵袭能力,RT-qPCR、Western Blot方法观察apelin/APJ信号通路相关因子VEGF、HIF-1α、PI3K等在各组干预细胞中的mRNA和蛋白表达。结果 CCK-8实验测得,缺氧环境能促进肺癌细胞增殖,益气养精方作用于A549、H1975的IC50分别为为1 mg·mL-1和0.5 mg·mL-1,且在常氧和缺氧微环境中均能抑制肺癌细胞增殖。Transwell实验结果显示,益气养精方组下室面细胞数量明显少于对照组（P < 0.01）,乏氧微环境中下室面细胞数明显多于对照组（P < 0.01）,加入益气养精方后亦可见减少。RT-qPCR结果显示,益气养精方在乏氧微环境中能不同程度下调血管生成相关因子HIF-1α、VEGF、APJ及Apelin的表达（P < 0.05）。Western Blot检测发现,益气养精方在乏氧环境中能使相关因子（VEGF、PI3K、APJ）的表达量明显下调,具有统计学意义。结论 益气养精方在乏氧微环境中能抑制肺癌细胞的增殖和侵袭,同时能通过Apelin/APJ通路对血管生成相关因子VEGF、HIF-1a、PI3K等起到一定的下调作用。
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| 关 键 词： | 中医药 非小细胞肺癌 Apelin/APJ 血管生成 |
| 收稿时间： | 2018-10-17 |
| 修稿时间： | 2019-11-27 |
Effects of Enhancing Qi and Nourishing Essence Formula on the Expression of Apelin in Lung Adenocarcinoma Cells under Hypoxic Condition |
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| Lu Wenfeng,Yang Xiwen,Dong Yun,Cui Qing,Cai Xiaoyue,Wang Yuhan,Zhang Ming,Deng Haibin and Xu Zhenye. Effects of Enhancing Qi and Nourishing Essence Formula on the Expression of Apelin in Lung Adenocarcinoma Cells under Hypoxic Condition[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2019, 21(11): 2531-2538 |
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| Authors: | Lu Wenfeng Yang Xiwen Dong Yun Cui Qing Cai Xiaoyue Wang Yuhan Zhang Ming Deng Haibin Xu Zhenye |
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| Affiliation: | Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Integrated TCM and Western Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
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| Abstract: | |
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| Keywords: | Traditional Chinese medicine Non-small lung cancer Apelin/APJ Angiogenesis. |
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