| 实时定量RT-PCR检测K562/A02细胞株bcr-abl融合基因mRNA水平 |
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| 引用本文： | 陈宝安,周桂娜,程坚,乔纯,吴雨洁,李建勇,丁家华,高冲,赵刚,王骏,鲍文,宋慧慧. 实时定量RT-PCR检测K562/A02细胞株bcr-abl融合基因mRNA水平[J]. 中国实验血液学杂志, 2011, 19(1): 40-43 |
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| 作者姓名： | 陈宝安 周桂娜 程坚 乔纯 吴雨洁 李建勇 丁家华 高冲 赵刚 王骏 鲍文 宋慧慧 |
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| 作者单位： | 1. 东南大学医学院附属中大医院血液科,江苏南京,210009
2. 江苏省人民医院血液科,江苏南京,210029 |
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| 基金项目： | 国家自然科学基金资助,编号39970832,30740062 |
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| 摘 要： | 本研究定量分析慢性髓系白血病耐阿霉素细胞株K562/A02细胞中bcr-abl融合基因的mRNA水平。收集对数生长期的K562/A02细胞,用TRIzoL提取RNA,用实时定量RT-PCR(RQ-RT-PCR)技术检测bcr-abl融合基因及内参基因abl mRNA表达水平。结果表明:RQ-RT-PCR技术分析103-107拷贝数的重复性良好,灵敏度达10-5;K562/A02细胞中bcr-abl融合基因为高表达,bcr-abl mRNA表达量大于100%。结论:实时定量PCR检测K562/A02细胞bcr/abl融合基因mRNA水平,敏感可靠,重复性好,有助于临床辅助监测慢性髓系白血病。
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| 关 键 词： | K562/A02细胞 bcr-abl融合基因 RQ-RT-PCR |
Detection of bcr-abl Fusion Gene mRNA Level in K562/A02 Cell Line by Real-time Quantitative RT-PCR |
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| CHEN Bao-An,ZHOU Gui-Na,CHENG Jian,QIAO Chun,WU Yu-Jie,LI Jian-Yong,DING Jia-Hua,GAO Chong,ZHAO Gang,WANG Jun,BAO Wen,SONG Hui-Hui. Detection of bcr-abl Fusion Gene mRNA Level in K562/A02 Cell Line by Real-time Quantitative RT-PCR[J]. Journal of experimental hematology, 2011, 19(1): 40-43 |
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| Authors: | CHEN Bao-An ZHOU Gui-Na CHENG Jian QIAO Chun WU Yu-Jie LI Jian-Yong DING Jia-Hua GAO Chong ZHAO Gang WANG Jun BAO Wen SONG Hui-Hui |
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| Affiliation: | CHEN Bao-An,ZHOU Gui-Na,CHENG Jian,QIAO Chun1,WU Yu-Jie1,LI Jian-Yong1,DING Jia-Hua,GAO Chong,ZHAO Gang,WANG Jun,BAO Wen,SONG Hui-Hui Department of Hematology,Zhongda Hospital,Southeast University Medical College,Nanjing 210009,Jiangsu Province,China,1Department of Hematology,Jiangsu Provincial Hospital,Nanjing 210029 |
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| Abstract: | |
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| Keywords: | K562/A02 cell bcr-abl fusion gene real-time quantitative RT-PCR |
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