高效液相色谱法测定复方磺胺甲噁唑分散片主要成分的含量

目的:采用高效液相色谱法测定复方磺胺甲噁唑分散片的含量.方法:以waters C18柱为分析柱;以水-乙腈-三乙胺(799:200:1)(用醋酸调pH至5.9±0.1)为流动相;检测波长:240 nm;流速:1.0 mLmin-1;柱温:30℃.结果:磺胺甲噁唑在162.1～810.4μg·ml-1范围内呈良好的线性,r=0.9999);平均回收率为98.83%(RSD=0.88%);甲氧苄啶在32.8～164 μg·ml-1范围内呈良好的线性,r=0.9999;平均回收率为98.48%(RSD=1.15%).结论:本方法操作简便,结果准确可靠,可用于复方磺胺甲噁唑分散片的质量控制.

Determination of main components in Compound Sulfamethoxazole Dispersible Tablets by HPLC

Objective：To establish a HPLC method for determining the content of main components in Compound Sulfamethoxazole Dispersible Tablets.Methods：The analytical column was Waters C18,the mobile phase was water-acetonitrile-triethylamine（799：200：1）.The detection wavelength was 240 nm.The flow rate was 1.0 mL·min-1.Results：The linear range of sulfamethoxazole（SMZ） was 162.1~810.4 μg·mL-1,r=0.999 9,the average recovery rate was 98.83%（RSD=0.88%）;the linear range of trimethoprim（TMP） was 32.8~164 μg·mL-1,r=0.999 9,the average recovery rate was 98.48%（RSD=1.15%）.Conclusion：The method is simple,rapid,accurate and repeatable,which can be used for the determination of SMZ and TMP in Compound Sulfamethoxazole Dispersible Tablets.