| 急性淋巴细胞白血病患儿骨髓间充质干细胞对K562/A02 细胞株耐药的影响 |
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| 引用本文： | 王昭霞,杨志敏,邹亚伟,李敏敏,陈福雄,钟帼钰,关镜明,卫凤桂,吴上志,何振涛,吴梓梁.急性淋巴细胞白血病患儿骨髓间充质干细胞对K562/A02 细胞株耐药的影响[J].中国实验血液学杂志,2011,19(1):19-23. |
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| 作者姓名： | 王昭霞 杨志敏 邹亚伟 李敏敏 陈福雄 钟帼钰 关镜明 卫凤桂 吴上志 何振涛 吴梓梁 |
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| 作者单位： | 1. 威海市文登中心医院儿科,山东威海,264400
2. 广州医学院第一附属医院儿科,广东广州,510120 |
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| 基金项目： | 广东省医学科学技术研究基金(2005288); 广州医学院博士启动基金(0706067) |
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| 摘 要： | 本研究探讨急性淋巴细胞白血病患儿骨髓间充质干细胞(mesenchymal stem cells,MSC)对K562/A02细胞耐药性的影响及其机制。从白血病患儿骨髓中分离、培养并鉴定MSC;建立K562/A02细胞株与MSC共培养的体系,应用AnnexinV-FITC检测一定浓度的阿霉素(ADM)对不同培养条件下K562/A02细胞凋亡的影响;RT-PCR检测K562/A02细胞中的凋亡基因家族中bcl-2和bax基因;荧光定量PCR检测多药耐药基因mdr1浓度。结果表明,单独培养组白血病细胞早期凋亡率为(8.38±0.29)%,而黏附培养组为(1.97±0.11)%,差异具有统计学意义(p<0.05)。相对于单独悬浮培养的K562/A02细胞,黏附共培养的K562/A02细胞bcl-2基因表达明显增强,bcl-2/bax比值显著增高;K562/A02单独悬浮培养组、黏附共培养组细胞中mdr1的水平比较无统计学差异(p>0.05)。结论:白血病患儿MSC能够使白血病细胞逃避药物的促凋亡作用,K562/A02对阿霉素产生耐药性可能与黏附共培养后bcl-2基因表达增强有关,而与其本身含有的mdr1基因无关。
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| 关 键 词： | 急性淋巴细胞白血病 间充质干细胞 K562/A02细胞 耐药 |
Effects of Acute Lymphoblastic Leukemia Children Bone Marrow Mesenchymal Stem Cells on Drug Resistance of K562/A02 Cell Line |
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| WANG Zhao-Xia,YANG Zhi-Min,ZOU Ya-Wei,LI Min-Min,CHEN Fu-Xiong,ZHONG Guo-Yu,GUAN Jing-Ming,WEI Feng-Gui,WU Shang-Zhi,HE Zheng-Tao,WU Zi-Liang.Effects of Acute Lymphoblastic Leukemia Children Bone Marrow Mesenchymal Stem Cells on Drug Resistance of K562/A02 Cell Line[J].Journal of Experimental Hematology,2011,19(1):19-23. |
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| Authors: | WANG Zhao-Xia YANG Zhi-Min ZOU Ya-Wei LI Min-Min CHEN Fu-Xiong ZHONG Guo-Yu GUAN Jing-Ming WEI Feng-Gui WU Shang-Zhi HE Zheng-Tao WU Zi-Liang |
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| Institution: | WANG Zhao-Xia,YANG Zhi-Min,ZOU Ya-Wei1,LI Min-Min1,CHEN Fu-Xiong1,ZHONG Guo-Yu,GUAN Jing-Ming1,WEI Feng-Gui1,WU Shang-Zhi1,HE Zheng-Tao1,WU Zi-Liang1 Department of Pediatrics,Wendeng Central Hospital,Weihai 264400,Shandong Province,China,1Department of Pediatrics,First Hospital,Guangzhou Medical College,Guangzhou 510120,Guangdong Province |
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| Abstract: | The aim of study was to investigate the effect of acute lymphoblastic leukemia(ALL) children bone marrow mesenchymal stem cells(MSC) on resistance of K562/A02 cells and its mechanism.MSC obtained from bone marrow of AL children were cultured and identified.The co-culture of MSC and K562/A02 and the culture of K562/A02 cell suspention alone was performed,of which 2 kinds of cells were treated with same concentration of adriamycin(ADM),and the rate of apoptosis was detected by flow cytometry,bcl-2 and bax of ... |
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| Keywords: | acute lymphoblastic leukemia mesenchymal stem cell K562/A02 cell drug resistance |
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