| Wt1反义寡核苷酸阻断白血病微小残留病wt1基因表达的体外研究 |
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| 引用本文： | 杨璐,曹悦鞍,彭朝胜,夏菁,张文洛,田力.Wt1反义寡核苷酸阻断白血病微小残留病wt1基因表达的体外研究[J].中国实验血液学杂志,2011,19(1):50-53. |
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| 作者姓名： | 杨璐 曹悦鞍 彭朝胜 夏菁 张文洛 田力 |
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| 作者单位： | 海军总医院特需医疗部,北京,100037 |
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| 摘 要： | 本研究通过wt1反义寡核苷酸(ASO)抑制wt1基因的表达,观察微小残留病模型及患者基因表达的变化。取56例复诊急性白血病完全缓解(CR)患者骨髓,用RT-PCR方法筛选出wt1基因表达阳性的骨髓标本。白血病细胞系微小残留病(MRD)模型及筛选出的wt1阳性急性白血病CR患者骨髓标本进行了细胞培养并用wt1ASO处理细胞,观察wt1基因表达的变化。结果表明:wt1基因的敏感性是10-3-10-4;56例急性白血病CR患者骨髓wt1基因的表达阳性率为16%。用wt1ASO处理wt1表达阳性的K562、HL-60细胞系MRD模型以及9例存在MRD的急性白血病CR患者骨髓,发现wt1ASO组有效的阻断了wt1基因的表达,而正义寡核苷酸组和对照组wt1基因的表达仍为阳性。结论:wt1ASO体外能够阻断白血病MRD中残留白血病细胞wt1基因的表达。
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| 关 键 词： | wt1 反义寡核苷酸 白血病 微小残留病 |
Minimal Residual Disease with wt1 Gene Expression Blocked by wt1 Antisense Oligonucleotides In Vitro |
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| YANG Lu,CAO Yue-An,PENG Chao-Sheng,XIA Jing,ZHANG Wen-Luo,TIAN Li.Minimal Residual Disease with wt1 Gene Expression Blocked by wt1 Antisense Oligonucleotides In Vitro[J].Journal of Experimental Hematology,2011,19(1):50-53. |
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| Authors: | YANG Lu CAO Yue-An PENG Chao-Sheng XIA Jing ZHANG Wen-Luo TIAN Li |
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| Institution: | YANG Lu,CAO Yue-An,PENG Chao-Sheng,XIA Jing,ZHANG Wen-Luo,TIAN Li Department of Special Necd,Navy General Hospital,Beijing 100037,China |
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| Abstract: | The aim of this study was to investigate the expression of wt1 gene and the changes of gene expression in minimal residual disease(MRD) models(K562,HL-60 cell lines) and acute leukemia(AL) patients through inhibiting the expression of wt1 gene by antisense oligonucleotides(ASO).The bone marrow(BM) of 56 AL patients with complete remission(CR) was collected,then the BM samples with positive expression of wt1 gene were screened by RT-PCR.The cells of MRD model and screened wt1 gene positive samples were cultu... |
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| Keywords: | wt1 antisense oligonucleotide leukemia minimal residual disease |
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