Overexpression of bcl-2 in transient abnormal myelopoiesis associated with Down syndrome |
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Authors: | K. Yoshioka S. Yamamoto N. Moriguchi H. Miyata K. Tsukiyama S. Isokawa F. Horiuchi T. Takemura |
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Affiliation: | (1) Department of Paediatrics, Kinki University School of Medicine, 377-2 Ohnohigashi, Osaka-sayama, 589-8511 Japan Tel.: +81-723-660221 Fax: +81-723-681566 e-mail: kzyoshio@med.kindai.ac.jp, JP;(2) The Third Department of Internal Medicine, Kinki University School of Medicine, Osaka-sayama, Japan, JP |
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Abstract: | Transient abnormal myelopoiesis (TAM) is a haematological complication found in Down syndrome. To determine the mechanisms of sustained proliferation of TAM cells, we studied the expression of apoptosis-related proteins, such as bcl-2, Fas (APO-1/CD95) and p-53, in peripheral blood cells from a new-born infant with Down syndrome and TAM. Using flow cytometry, peripheral blood mononuclear cells (PBMCs), consisting mostly of blast cells, showed marked expression of bcl-2 protein but not of Fas or p-53 products. DNA gel electrophoresis of PBMCs, cultured in the absence of serum factors, revealed no marked fragmentation. Our findings suggest that bcl-2 overexpression may be associated with prolonged cell survival of TAM cells. Received: 8 March 1999 / Accepted: 9 November 1999 |
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Keywords: | Down syndrome Transient abnormal myelopoiesis Apoptosis bcl-2 |
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