软脂酸对体外原代培养大鼠胰岛细胞凋亡作用的实验研究

目的 观察软脂酸诱导体外原代培养胰岛细胞的凋亡，初步探讨软脂酸在2型糖尿病发病中的机制。方法 应用分离的体外单层培养SD大鼠胰岛细胞，与不同浓度软脂酸(分别为0、0．125、0．250、0．500mmol／L)共同孵育48h，用放免法测定培养液中胰岛素含量，PI／Hoechst33342双染法荧光显微镜观察胰岛细胞凋亡的形态变化。结果 0．250、0．5mmol／L软脂酸可抑制高糖刺激下的胰岛素分泌，促进胰岛细胞的凋亡。结论 软脂酸可诱导胰岛细胞凋亡，凋亡程度与游离软脂酸浓度有关。

Apoptosis-inducing effect of palmitic acids on rat pancreatic islet cells in primary culture: a preliminary study]

OBJECTIVE: To investigate the in vitro apoptosis-inducing effect of palmitic acid on pancreatic islet cells in primary culture, thereby to understand the role of palmitic acid in the pathogenesis of type 2 diabetes mellitus. METHOD: SD rat pancreatic islet cells were isolated and cultured in monolayer in vitro followed by incubation with stepwise diluted palmitic acid (0, 0.125, 0.25 mmol/L and 0.5 mmol/L respectively), and the insulin concentrations in the culture medium were determined by radio immunological methods. Morphological observation with a fluorescence microscope was conducted after double staining of the cells with PI/Hoechst 33342. RESULTS: The glucose-stimulated insulin secretion was inhibited by palmitic acid at the concentration of 0.25 and 0.5 mmol/L, which also induced obvious cell apoptosis. CONCLUSION: Palmitic acid is capable of inducing islet cell apoptosis in a dose-dependent manner.