| 血清代谢组学技术在2型糖尿病肾病早期诊断中的应用 |
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| 引用本文： | 董哲毅,周伟,华参,高翔,蔡厚安,华振浩,付莉莉,梅长林.血清代谢组学技术在2型糖尿病肾病早期诊断中的应用[J].山东医药,2010,50(9):19-22. |
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| 作者姓名： | 董哲毅 周伟 华参 高翔 蔡厚安 华振浩 付莉莉 梅长林 |
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| 作者单位： | 1. 中国人民解放军第309医院,北京,100091
2. 第二军医大学附属长征医院肾脏病研究所 |
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| 摘 要： | 目的探讨基于液质联用（LC／MS）的代谢组学技术在2型糖尿病肾病动物模型KK—Ay小鼠不同病程的诊断应用价值。方法试验组KK-Ay小鼠和对照组C57BL／6J小鼠各15只，从8周龄开始，每4周收集24h尿液测尿微量白蛋白，乙醚麻醉后空腹采血，分离血清检测空腹血糖、甘油三酯、尿素氮、肌酐、白蛋白等，同时对血清样品预处理进行LC／MS检测及代谢组学分析。第8、12、20周龄两组各处死3只小鼠，灌洗取肾后进行病理学检查。结果12周龄后KK—Ay小鼠空腹血糖、甘油三酯、尿微量白蛋白与C57BL／6J小鼠有统计学差异（P〈0．01）。KK—Ay小鼠12周龄系膜基质增加明显，20周龄出现基底膜增厚等病理改变。代谢组学主成份分析显示，各周龄KK—Ay小鼠与C57BIM6J小鼠血清代谢图谱差异显著，早于血生化和肾脏病理变化，代谢物模式的经时变化轨迹图能够反映2型糖尿病肾病的进展规律。结论基于LC／MS的代谢组学技术对动物血清的检测分析能准确区分各周龄试验组和对照组小鼠，并揭示病程进展规律，其作为一种辅助手段，可能在2型糖尿病肾病的早期诊断中具有应用前景。
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| 关 键 词： | 糖尿病，2型 糖尿病肾病 代谢组学 液质联用 主成份分析 |
Metabomomic study of blood serum in early diagnosis of type 2 diabetic nephrology |
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| DONG Zhe-yi,ZHOU Wei,HUA Can,GAO Xiang,CAI Hou-an,HUA Zhen-hao,FU Li-li,MEI Chang-lin.Metabomomic study of blood serum in early diagnosis of type 2 diabetic nephrology[J].Shandong Medical Journal,2010,50(9):19-22. |
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| Authors: | DONG Zhe-yi ZHOU Wei HUA Can GAO Xiang CAI Hou-an HUA Zhen-hao FU Li-li MEI Chang-lin |
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| Institution: | DONG Zhe-yi1,ZHOU Wei,HUA Can,GAO Xiang,CAI Hou-an,HUA Zhen-hao,FU Li-li,MEI Chang-lin (1 Chinese People\'s Liberation Army 309 Hospital,Beijing 100091,P.R.China) |
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| Abstract: | Objective To access the capability of LC/MS based metabonomics in the evaluation of different course of diabetic nephropathy in KK-Ay mice.Methods Thirty male KK-Ay mice and C57BL/6J mice each were studied at the age of eight weeks.A 24-hour urine collection was made for the tests of protein urine every four weeks.Blood serum were collected for biochemical and LC/MS analysis.Three mice of each group were sacrificed at the age of 8,12,20 weeks.Renal tissue was observed to assess pathologic change.Results The... |
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| Keywords: | diabetes mellitus type 2 diabetic nephropathy Metabomomic Liquid Chromatography-mass Spectrometry principal component analysis |
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