| 成年大鼠海马CAI区锥体细胞的急性分离方法 |
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| 引用本文： | 张庆国,雷洪伊,徐世元. 成年大鼠海马CAI区锥体细胞的急性分离方法[J]. 中国现代医学杂志, 2004, 14(11): 6-9 |
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| 作者姓名： | 张庆国 雷洪伊 徐世元 |
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| 作者单位： | 第一军医大学珠江医院,麻醉科,广东,广州,510282 |
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| 摘 要： | 目的建立一种膜片钳技术(patch clamp technique,PCT)的成年大鼠海马CAI区锥体细胞(adultra hippocampal CAI pyramidal neuron,ARHCPN)的急性分离方法.方法采用酶加机械分离的方法制备ARHCPN,用相差显微镜观察其形态特征,并用PCT的细胞贴附模式记录L-型Ca2 通道的单通道电话动.结果所分离的ARHCPN不仅形成学特征保存完好,而且保存了L型Ca2 通道的电生理学特性.结论成功地建立了一种简单、快速、可靠的适用于膜片钳单通道记录的ARHCPN的急性分离方法.
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| 关 键 词： | 大鼠 海马 锥体细胞 急性分离 膜片钳 |
method of acute isolation of CAI pyramidal neurons in hippocampus of adult rats |
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| Abstract. method of acute isolation of CAI pyramidal neurons in hippocampus of adult rats[J]. China Journal of Modern Medicine, 2004, 14(11): 6-9 |
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| Authors: | Abstract |
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| Abstract: | Objective:To establish a method of acute isolation of adult rat hippocampal CAI pyramidal neurons(ARHCPN)suitable for patch clamp technique(PCT).Methods:ARHCPN were obtained by a combi-nation of mechanical and enzymatic means.The isolated neurons were identified under a phasic contrast mi-croscope,and cell-attached mode was used to record L-type calcium channel currents.Results:The isolated ARHCPN exhibited the well-kept proximal dendritic structures with clean surface,and preserved electrophysi-ological properties of L-type calcium channel.Conclusions:A simple,fast and credible method of acute iso-lation of ARHCPN suitable for single channel recording is successfully founded. |
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| Keywords: | rat hippocampus pyramidal neuron acute isolation patch clamp |
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