Vaccine derived bi- and multi-recombinant Sabin strains |
| |
Authors: | Eleni Paximadi Ioannis Karakasiliotis Eugenia Bolanaki Aris Krikelis Panayotis Markoulatos |
| |
Institution: | (1) Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, Ploutonos 26 & Aiolou, 41221 Larissa, Greece;(2) Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece;(3) Department of Virology, Imperial College London, London, UK;(4) Division of Molecular Biosciences, Imperial College London, London, UK |
| |
Abstract: | A retrospective analysis of five Sabin intertypic recombinant strains, isolated from human feacal specimens during the time
period 1978–1985 in Greece, was performed by RT-PCR, Restriction Fragment Length Polymorphism (R.F.L.P.) and sequencing. Of
the studied strains, three (EPA, EPB, EPC) were found to be bi-recombinant Sabin3/Sabin2/Sabin3 (S3/S2/S3), one strain was
characterized as a probable S3/S2- CAV18 or CAV21-S2/S1 multi-recombinant (EDP11) and one was identified as a tripartite one
S3/S2/S1 (EDP12). Samples EPA, EPB and EPC presented a common recombination junction in the 2C genomic region. Moreover, strains
EPA and EPB shared also the second recombination site in the 3D genomic region, whereas the second recombination of EPC was
also determined in 3D but in a different nucleotide position. Strains EDP11 and EDP12 presented both identical recombination
motifs and recombination sites. The first was detected in the 2C genomic region and the second in the 3D region. Strain EDP11
presented an interesting feature since a sequence of 120 nucleotides seems to have derived from a member of human enteroviruses
species C (CAV18 or CAV21). This finding is of great importance, considering that this strain (EDP11) was isolated from an
area and time period, where no Coxsackie A virus or poliovirus epidemics occurred. Our study underlines the role of specific
positions and motifs of the poliovirus genomic sequences involved in recombination events and prompts that Coxsackie A viruses
belonging to human enterovirus species C (genetically closely related to PV) are considered as the possible counterparts of
the recombination. |
| |
Keywords: | Enteroviruses Sabin Recombination PCR Sequencing |
本文献已被 PubMed SpringerLink 等数据库收录! |
|